Journal article
Host-derived enterocytes in intestinal grafts
Transplantation, Vol.74(1), pp.120-122
2002
DOI: 10.1097/00007890-200207150-00020
PMID: 12134109
Abstract
Replacement of donor lymphoid tissue by lymphocytes of recipient origin is an established phenomenon in small bowel transplants. However, replacement of donor epithelial cells of bowel grafts by host cells has not been demonstrated. The objective of our study was to determine whether donor enterocytes are replaced by host-derived enterocytes in the intestinal allograft. Graft biopsy specimens, obtained from five human male recipients of female intestine, were examined for the presence of male enterocytes. The biopsies dated from 90 to 770 days posttransplant. Formalin-fixed 3-μm specimen sections were stained for X and Y chromosomes by fluorescent in situ hybridization technique. Fluorescent microscopy of the stained sections identified male enterocytes in four patients, with a percentage of male cells ranging from 0.09% to 0.26% of the total enterocyte mass. Using the fluorescent in situ hybridization technique, we demonstrated the presence of host-derived male (XY) enterocytes in the female intestinal graft.
Details
- Title: Subtitle
- Host-derived enterocytes in intestinal grafts
- Creators
- Panagiotis TRYPHONOPOULOS - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesMichael ICARDI - Department of Pathology, University of Miami School of Medicine, Miami, Florida, United StatesGeorge MICHALOPOULOS - Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United StatesJoshua MILLER - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesAndreas TZAKIS - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesShashikumar SALGAR - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesPhillip RUIZ - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesTatsuya FUKUMORI - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesCarlos GANDIA - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesKonstantinos BOUKAS - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesTomoaki KATO - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesViolet ESQUENAZI - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United StatesCamillo RICORDI - Department of Surgery, University of Miami School of Medicine, Miami, Florida, United States
- Resource Type
- Journal article
- Publication Details
- Transplantation, Vol.74(1), pp.120-122
- Publisher
- Lippincott; Hagerstown, MD
- DOI
- 10.1097/00007890-200207150-00020
- PMID
- 12134109
- ISSN
- 0041-1337
- eISSN
- 1534-6080
- Language
- English
- Date published
- 2002
- Academic Unit
- Pathology
- Record Identifier
- 9984047704702771
Metrics
15 Record Views