Journal article
Host factor Rab11a is critical for efficient assembly of influenza A virus genomic segments
PLoS pathogens, Vol.17(5), pp.e1009517-e1009517
05/2021
DOI: 10.1371/journal.ppat.1009517
PMCID: PMC8136845
PMID: 33970958
Abstract
It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion; however, the role of host factors in genome assembly is not completely understood. To evaluate the significance of cellular factors in genome assembly, we generated a reporter virus carrying a tetracysteine tag in the NP gene (NP-Tc virus) and assessed the dynamics of vRNP localization with cellular components by fluorescence microscopy. At early time points, vRNP complexes were preferentially exported to the MTOC; subsequently, vRNPs associated on vesicles positive for cellular factor Rab11a and formed distinct vRNP bundles that trafficked to the plasma membrane on microtubule networks. In Rab11a deficient cells, however, vRNP bundles were smaller in the cytoplasm with less co-localization between different vRNP segments. Furthermore, Rab11a deficiency increased the production of non-infectious particles with higher RNA copy number to PFU ratios, indicative of defects in specific genome assembly. These results indicate that Rab11a+ vesicles serve as hubs for the congregation of vRNP complexes and enable specific genome assembly through vRNP:vRNP interactions, revealing the importance of Rab11a as a critical host factor for influenza A virus genome assembly.
Details
- Title: Subtitle
- Host factor Rab11a is critical for efficient assembly of influenza A virus genomic segments
- Creators
- Julianna Han - University of ChicagoKetaki Ganti - Emory UniversityVeeresh Kumar Sali - University of IowaCarly Twigg - University of IowaYifeng Zhang - University of IowaSenthamizharasi Manivasagam - University of IowaChieh-Yu Liang - University of IowaOlivia A Vogel - University of IowaIris Huang - University of ChicagoShanan N Emmanuel - University of ChicagoJesse Plung - University of ChicagoLillianna Radoshevich - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, United States of AmericaJasmine T Perez - University of ChicagoAnice C Lowen - Emory UniversityBalaji Manicassamy - University of Iowa
- Resource Type
- Journal article
- Publication Details
- PLoS pathogens, Vol.17(5), pp.e1009517-e1009517
- DOI
- 10.1371/journal.ppat.1009517
- PMID
- 33970958
- PMCID
- PMC8136845
- NLM abbreviation
- PLoS Pathog
- ISSN
- 1553-7366
- eISSN
- 1553-7374
- Publisher
- Public Library of Science
- Grant note
- R25 GM066522 / NIGMS NIH HHS R01 AI127775 / NIAID NIH HHS R35 GM137961 / NIGMS NIH HHS HHSN272201400004C / NIAID NIH HHS T32 GM007183 / NIGMS NIH HHS R01 AI123359 / NIAID NIH HHS R01 AI125268 / NIAID NIH HHS
- Language
- English
- Date published
- 05/2021
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984230423102771
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