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Host modification of the adherence properties of Chlamydia trachomatis
Journal article   Open access   Peer reviewed

Host modification of the adherence properties of Chlamydia trachomatis

Subir K Bose and P. C GOSWAMI
Journal of general microbiology, Vol.132(6), pp.1631-1639
1986
DOI: 10.1099/00221287-132-6-1631
PMID: 3806051
url
https://doi.org/10.1099/00221287-132-6-1631View
Published (Version of record) Open Access

Abstract

The adherence of Chlamydia trachomatis LGV440(L1) to human HeLa 229 and mouse McCoy cells was stimulated by the lectin wheat germ agglutinin (WGA) and inhibited by the sugars N-acetyl-D-glucosamine, N-acetyl-D-galactosamine and chitobiose, but only when the chlamydiae had been passaged several times in HeLa cells. After passage in McCoy cells, the lectin and the sugars elicited little response. The non-LGV serovar UW-31(K), however, differed from LGV440(L1) in that, regardless of passage, the lectin and sugar effects were observed only in HeLa cells. Affinity chromatography on WGA-agarose confirmed that HeLa-grown LGV440(L1) bound to a significantly greater extent relative to McCoy-grown chlamydiae. In addition, participation of heterogeneous chlamydial ligands was suggested by the observation that the adherence of heated (60 degrees C, 5 min) UW-31(K) to HeLa cells at 37 degrees C was not inhibited at all, but at 5 degrees C, the adherence rate was greatly reduced, indicating the participation of heat-stable as well as heat-labile ligands. These data are interpreted to indicate that the passage history of C. trachomatis results in the acquisition of altered surface components that participate in the initial interaction of the bacterium with the host.
 Bacteriology Microbiology Fundamental and applied biological sciences. Psychology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Biological and medical sciences

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