Journal article
Host passage-dependent wheat germ agglutinin-binding proteins of Chlamydia trachomatis
FEMS microbiology letters, Vol.81(1), pp.53-56
06/01/1991
DOI: 10.1111/j.1574-6968.1991.tb04711.x
PMID: 1874403
Abstract
Wheat germ agglutinin (WGA)-binding proteins in the highly purified elementary bodies (EB) of Chlamydia trachomatis were detected by an ELISA-like assay of immobilized EB. Trypsin-sensitivity of the WGA-binding moieties was detected only in the chlamydiae grown in HeLa cells. A nonionic detergent, beta-octyl-D-glucopyranoside (OG), was used to extract proteins from the purified EB. Proteins in the extract were resolved by SDS-PAGE and probed with 125I-labelled WGA. Several proteins reacted with WGA in a specific manner. Some of these WGA-binding proteins in the EB of serovars L1 and E exhibited altered molecular mass after adaptation in the two alternate hosts, HeLa and McCoy cells. These results suggest that WGA-binding proteins exist in chlamydiae.
Details
- Title: Subtitle
- Host passage-dependent wheat germ agglutinin-binding proteins of Chlamydia trachomatis
- Creators
- P C Goswami - Department of Microbiology, St. Louis University School of Medicine, MO 63104-1028E VretouS K Bose
- Resource Type
- Journal article
- Publication Details
- FEMS microbiology letters, Vol.81(1), pp.53-56
- DOI
- 10.1111/j.1574-6968.1991.tb04711.x
- PMID
- 1874403
- NLM abbreviation
- FEMS Microbiol Lett
- ISSN
- 0378-1097
- eISSN
- 1574-6968
- Publisher
- England
- Grant note
- AI-19575 / NIAID NIH HHS
- Language
- English
- Date published
- 06/01/1991
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047856502771
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