Journal article
House dust microbiota in relation to adult asthma and atopy in a US farming population
Journal of allergy and clinical immunology, Vol.147(3), pp.910-920
2021
DOI: 10.1016/j.jaci.2020.06.013
PMCID: PMC7770060
PMID: 32615170
Abstract
Bacterial exposure from house dust has been associated with asthma and atopy in children but whether these relationships are present in adults remains unclear.
We sought to examine associations of house dust microbiota with adult asthma, atopy, and hay fever.
Vacuumed bedroom dust samples from the homes of 879 participants (average age, 62 years) in the Agricultural Lung Health Study, a case-control study of asthma nested within a farming cohort, were subjected to 16S rRNA amplicon sequencing to characterize bacterial communities. We defined current asthma and hay fever using questionnaires and current atopy by blood specific IgE level > 0.70 IU/mL to 1 or more of 10 common allergens. We used linear regression to examine whether overall within-sample bacterial diversity differed by outcome, microbiome regression-based kernel association test to evaluate whether between-sample bacterial community compositions differed by outcome, and analysis of composition of microbiomes to identify differentially abundant bacterial taxa.
Overall diversity of bacterial communities in house dust was similar by asthma status but was lower (P < .05) with atopy or hay fever. Many individual bacterial taxa were differentially abundant (false-discovery rate, <0.05) by asthma, atopy, or hay fever. Several taxa from Cyanobacteria, Bacteroidetes, and Fusobacteria were more abundant with asthma, atopy, or hay fever. In contrast, several taxa from Firmicutes were more abundant in homes of individuals with adequately controlled asthma (vs inadequately controlled asthma), individuals without atopy, or individuals without hay fever.
Microbial composition of house dust may influence allergic outcomes in adults.
Details
- Title: Subtitle
- House dust microbiota in relation to adult asthma and atopy in a US farming population
- Creators
- Mi Kyeong Lee - Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Research Triangle Park, NCAnnah B Wyss - Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Research Triangle Park, NCMegan U Carnes - Genomics in Public Health and Medicine Center, Biostatistics and Epidemiology Division, RTI International, Research Triangle Park, NCMarie Richards - Westat, Durham, NCChristine G Parks - Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Research Triangle Park, NCLaura E Beane Freeman - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, Rockville, MdPeter S Thorne - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IowaDavid M Umbach - Biostatistics and Computational Biology Branch, NIEHS, NIH, DHHS, Research Triangle Park, NCM. Andrea Azcarate-Peril - Department of Medicine and Microbiome Core, Center for Gastrointestinal Biology and Disease, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NCShyamal D Peddada - Department of Biostatistics, University of Pittsburgh, Pittsburgh, PaStephanie J London - Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Research Triangle Park, NC
- Resource Type
- Journal article
- Publication Details
- Journal of allergy and clinical immunology, Vol.147(3), pp.910-920
- DOI
- 10.1016/j.jaci.2020.06.013
- PMID
- 32615170
- PMCID
- PMC7770060
- NLM abbreviation
- J Allergy Clin Immunol
- ISSN
- 0091-6749
- eISSN
- 1097-6825
- Publisher
- Elsevier Inc
- Grant note
- American Recovery and Reinvestment Act P30 ES005605 / NIH (https://doi.org/10.13039/100000002) University of North Carolina (https://doi.org/10.13039/100006808) National Institutes of Health (https://doi.org/10.13039/100000002) Z01-CP010119B / National Cancer Institute (https://doi.org/10.13039/100000054) P30 DK34987 / National Institute of Diabetes and Digestive and Kidney Diseases (https://doi.org/10.13039/100000062) Z01 ES043012, Z01-ES049030; Z01-ES102385 / National Institute of Environmental Health Sciences (https://doi.org/10.13039/100000066)
- Language
- English
- Date published
- 2021
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9984001079202771
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