Journal article
How Should Apples Be Prepared for a Fruit Salad? A Guided Inquiry Physical Chemistry Experiment
Journal of chemical education, Vol.97(12), pp.4475-4481
12/08/2020
DOI: 10.1021/acs.jchemed.0c00517
Abstract
In this guided inquiry experiment, students extract catecholase enzyme from apples to catalyze the oxidation of catechol. They follow the reaction using the UV-vis absorbance of the p-benzophenone produced to determine the Michaelis-Menten kinetic parameters. Students make selected experimental choices within a structured framework such as selecting the apple varietal, the pH of the reaction mixture, and the reaction inhibitor. The experiment has been tested at multiple universities in physical chemistry laboratory courses with both large and small enrollments. We describe the experiment and its implementation in both synchronously and asynchronously taught courses.
Details
- Title: Subtitle
- How Should Apples Be Prepared for a Fruit Salad? A Guided Inquiry Physical Chemistry Experiment
- Creators
- Renée S Cole - Chemistry Department, University of Iowa, W331 Chemistry Building, Iowa City, Iowa 52242, United StatesMarc Muniz - Chemistry and Chemical Biology, Rutgers The State University of New Jersey, 123 Bevier Road, Piscataway, New Jersey 08854, United StatesErica Harvey - Chemistry, Fairmont State University, 1201 Locust Avenue, Fairmont, West Virginia 26554, United StatesRobert Sweeney - Center for Excellence in Teaching, University of Southern California, 3601 West Way, GFS227, Los Angeles, California 90089-0001, United StatesSally Hunnicutt - Chemistry, Virginia Commonwealth University, 1001 West Main Street, Richmond, Virginia 23284-2006, United States
- Resource Type
- Journal article
- Publication Details
- Journal of chemical education, Vol.97(12), pp.4475-4481
- DOI
- 10.1021/acs.jchemed.0c00517
- ISSN
- 0021-9584
- eISSN
- 1938-1328
- Grant note
- DOI: 10.13039/100000172, name: Division of Undergraduate Education, award: DUE-1044624, DUE-1726066, DUE-1726071
- Language
- English
- Date published
- 12/08/2020
- Academic Unit
- Chemistry
- Record Identifier
- 9984216683102771
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