Journal article
Hox11 paralogous genes are essential for metanephric kidney induction
Genes & development, Vol.16(11), pp.1423-1432
06/01/2002
DOI: 10.1101/gad.993302
PMCID: PMC186320
PMID: 12050119
Abstract
The mammalian
Hox
complex is divided into four linkage groups containing 13 sets of paralogous genes. These paralogous genes have retained functional redundancy during evolution. For this reason, loss of only one or two
Hox
genes within a paralogous group often results in incompletely penetrant phenotypes which are difficult to interpret by molecular analysis. For example, mice individually mutant for
Hoxa11
or
Hoxd11
show no discernible kidney abnormalities.
Hoxa11/Hoxd11
double mutants, however, demonstrate hypoplasia of the kidneys. As described in this study, removal of the last
Hox11
paralogous member,
Hoxc11
, results in the complete loss of metanephric kidney induction. In these triple mutants, the metanephric blastema condenses, and expression of early patterning genes,
Pax2
and
Wt1
, is unperturbed.
Eya1
expression is also intact.
Six2
expression, however, is absent, as is expression of the inducing growth factor,
Gdnf
. In the absence of
Gdnf
, ureteric bud formation is not initiated. Molecular analysis of this phenotype demonstrates that
Hox11
control of early metanephric induction is accomplished by the interaction of
Hox11
genes with the
pax-eya-six
regulatory cascade, a pathway that may be used by
Hox
genes more generally for the induction of multiple structures along the anteroposterior axis.
Details
- Title: Subtitle
- Hox11 paralogous genes are essential for metanephric kidney induction
- Creators
- Deneen M. Wellik - University of UtahPatrick J. Hawkes - University of UtahMario R. Capecchi - University of Utah
- Resource Type
- Journal article
- Publication Details
- Genes & development, Vol.16(11), pp.1423-1432
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/gad.993302
- PMID
- 12050119
- PMCID
- PMC186320
- ISSN
- 0890-9369
- eISSN
- 1549-5477
- Language
- English
- Date published
- 06/01/2002
- Academic Unit
- Surgery
- Record Identifier
- 9984321860702771
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