Journal article
Human β-Defensins 2 and 3 Demonstrate Strain-Selective Activity against Oral Microorganisms
Journal of clinical microbiology, Vol.42(3), pp.1024-1029
03/2004
DOI: 10.1128/JCM.42.3.1024-1029.2004
PMCID: PMC356847
PMID: 15004048
Abstract
Human β-defensins 2 and 3 (HBD-2 and HBD-3) are inducible peptides present at sites of infection in the oral cavity. A few studies have reported broad-spectrum antimicrobial activity for both peptides. However, no comprehensive study has thoroughly investigated their potential against oral pathogens. The purpose of this study was to test the effectiveness of HBD-2 and HBD-3 against a collection of oral organisms (
Actinobacillus actinomycetemcomitans
,
Fusobacterium nucleatum
,
Porphyromonas gingivalis
,
Peptostreptococcus micros
,
Actinomyces naeslundii
,
Actinomyces israelii
,
Streptococcus sanguis
,
Streptococcus mutans
,
Candida tropicalis
,
Candida parapsilosis
,
Candida krusei
,
Candida glabrata
, and
Candida albicans
). Radial diffusion assays were used to test HBD-2 and HBD-3 activities against at least three strains of each species. There was significant variability in MICs, which was strain specific rather than species specific. MICs ranged from 3.9 to >250 μg/ml for HBD-2 and from 1.4 to >250 μg/ml for HBD-3. HBD-3 demonstrated greater antimicrobial activity and was effective against a broader array of organisms. Overall, aerobes were 100% susceptible to HBD-2 and HBD-3, whereas only 21.4 and 50% of the anaerobes were susceptible to HBD-2 and HBD-3, respectively. HBD-2 and HBD-3 also demonstrated strain-specific activity against the
Candida
species evaluated. Interestingly, an association between HBD-2 and HBD-3 activities was noted. This suggests that the two peptides may have similar mechanisms yet utilize distinct pathways. The lack of activity against specific anaerobic strains and
Candida
warrants further investigation of the potential resistance mechanisms of these organisms. Finally, the significant variability between strains underlies the importance of testing multiple strains when evaluating activities of antimicrobial peptides.
Details
- Title: Subtitle
- Human β-Defensins 2 and 3 Demonstrate Strain-Selective Activity against Oral Microorganisms
- Creators
- Sophie Joly - Dows Institute for Dental ResearchConnie Maze - Dows Institute for Dental ResearchPaul B McCray - Dows Institute for Dental ResearchJanet M Guthmiller - Dows Institute for Dental Research
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.42(3), pp.1024-1029
- DOI
- 10.1128/JCM.42.3.1024-1029.2004
- PMID
- 15004048
- PMCID
- PMC356847
- NLM abbreviation
- J Clin Microbiol
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 03/2004
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093322302771
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