Journal article
Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
Scientific reports, Vol.10(1), 10286
12/2020
DOI: 10.1038/s41598-020-67175-3
PMID: 32581231
Abstract
Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and their precursors (pre-adipocytes) secrete factors that affect human dermal fibroblast behavior. We found that both adipocyte and pre-adipocyte conditioned medium induced the migration of fibroblasts, but only adipocyte conditioned medium induced fibroblast differentiation into a highly contractile, collagen producing myofibroblast phenotype. Furthermore, adipocyte mediated myofibroblast induction occurred through a TGF-β independent mechanism. Our findings contribute to a better understanding on the involvement of adipose tissue in wound healing, and may help to uncover and develop fat-related wound healing treatments.
Details
- Title: Subtitle
- Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
- Creators
- Mariam Y El-HattabYoshiaki NagumoFrancoise A GourroncAloysius J KlingelhutzJames A AnkrumEdward A Sander
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.10(1), 10286
- DOI
- 10.1038/s41598-020-67175-3
- PMID
- 32581231
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Language
- English
- Date published
- 12/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Microbiology and Immunology; Orthopedics and Rehabilitation; Radiation Oncology; Craniofacial Anomalies Research Center; Neuroscience and Pharmacology; Chemical and Biochemical Engineering
- Record Identifier
- 9984000930802771
Metrics
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