Journal article
Human Adipose-Derived Mesenchymal Stromal/Stem Cells Remain Viable and Metabolically Active Following Needle Passage
PM & R, Vol.8(9), pp.844-854
09/2016
DOI: 10.1016/j.pmrj.2016.01.010
PMCID: PMC6724701
PMID: 26826615
Abstract
To assess the biological effects of passage through clinically relevant needles on the viability and metabolic activity of culture-expanded, human adipose tissue-derived mesenchymal stromal/stem cells (AMSCs).
Prospective observational pilot study.
Academic medical center.
Patient-derived clinical-grade culture expanded AMSCs.
AMSCs were passed through syringes without a needle attached (control), with an 18-gauge (25.4-mm) needle attached and with a 30-gauge (19-mm) needle attached at a constant injection flow rate and constant cell concentrations. Each injection condition was completed in triplicate.
Cell number and viability, proliferative capacity, metabolic activity, and acute gene expression as measured by cell counts, mitochondrial activity, and quantitative real time reverse-transcription polymerase chain reaction on day 0 (immediately), day 1, and day 4 after injection.
AMSC viability was not significantly affected by injection, and cells proliferated normally regardless of study group. Postinjection, AMSCs robustly expressed both proliferation markers and extracellular matrix proteins. Stress-response mRNAs were markedly but transiently increased independently of needle size within the first day in culture postinjection.
Human, culture-expanded AMSCs maintain their viability, proliferative capacity, and metabolic function following passage through needles as small as 30-gauge at constant flow rates of 4 mL/min, despite an early, nonspecific stress/cytoprotective response. These initial findings suggest that culture-expanded AMSCs should tolerate the injection process during most cell-based therapeutic interventions.
Details
- Title: Subtitle
- Human Adipose-Derived Mesenchymal Stromal/Stem Cells Remain Viable and Metabolically Active Following Needle Passage
- Creators
- Kentaro Onishi - Mayo Clinic in ArizonaDakota L Jones - Mayo Clinic in ArizonaScott M Riester - Mayo Clinic in ArizonaEric A Lewallen - National Institute of Arthritis and Musculoskeletal and Skin DiseasesDavid G Lewallen - StrykerJacob L Sellon - Mayo Clinic in ArizonaAllan B Dietz - Mayo Clinic in ArizonaWenchun Qu - Mayo Clinic in ArizonaAndre J van Wijnen - National Institute of Arthritis and Musculoskeletal and Skin DiseasesJay Smith - Mayo Clinic in Arizona
- Resource Type
- Journal article
- Publication Details
- PM & R, Vol.8(9), pp.844-854
- DOI
- 10.1016/j.pmrj.2016.01.010
- PMID
- 26826615
- PMCID
- PMC6724701
- NLM abbreviation
- PM R
- ISSN
- 1934-1482
- eISSN
- 1934-1563
- Grant note
- T32 AR056950 / NIAMS NIH HHS R01 AR049069 / NIAMS NIH HHS
- Language
- English
- Date published
- 09/2016
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984949263302771
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