Journal article
Human Nedd4 interacts with the human epithelial Na+ channel: WW3 but not WW1 binds to Na+-channel subunits
The Biochemical journal, Vol.345(3), pp.503-509
02/01/2000
DOI: 10.1042/0264-6021:3450503
PMCID: PMC1220784
PMID: 10642508
Abstract
The epithelial Na(+) channel (ENaC) regulates Na(+) absorption in epithelial tissues including the lung, colon and sweat gland, and in the distal nephrons of the kidney. When Na(+)-channel function is disrupted, salt and water homoeostasis is affected. The cytoplasmic regions of the Na(+)-channel subunits provide binding sites for other proteins to interact with and potentially regulate Na(+)-channel activity. Previously we showed that a proline-rich region of the alpha subunit of the Na(+) channel bound to a protein of 116 kDa from human lung cells. Here we report the identification of this protein as human Nedd4, a ubiquitin-protein ligase that binds to the Na(+)-channel subunits via its WW domains. Further, we show that WW domains 2, 3 and 4 of human Nedd4 bind to the alpha, beta and gamma Na(+)-channel subunits but not to a mutated beta subunit. In addition, when co-expressed in Xenopus oocytes, human Nedd4 down-regulates Na(+)-channel activity.
Details
- Title: Subtitle
- Human Nedd4 interacts with the human epithelial Na+ channel: WW3 but not WW1 binds to Na+-channel subunits
- Creators
- Tracy J FARRSarah J CODDINGTON-LAWSONPeter M SNYDERFiona J MCDONALD
- Resource Type
- Journal article
- Publication Details
- The Biochemical journal, Vol.345(3), pp.503-509
- DOI
- 10.1042/0264-6021:3450503
- PMID
- 10642508
- PMCID
- PMC1220784
- NLM abbreviation
- Biochem J
- ISSN
- 0264-6021
- eISSN
- 1470-8728
- Language
- English
- Date published
- 02/01/2000
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Medicine Administration; Internal Medicine
- Record Identifier
- 9984025694002771
Metrics
22 Record Views