Journal article
Human Neutrophils Express the Prostaglandin G/H Synthase 2 Gene When Stimulated with Bacterial Lipopolysaccharide
Clinical immunology and immunopathology, Vol.87(3), pp.304-308
06/1998
DOI: 10.1006/clin.1998.4545
PMID: 9646841
Abstract
Human blood neutrophils (PMN) rapidly release arachidonic acid (AA) from cellular phospholipids when stimulatedin vitrowith a variety of inflammatory agonists. Free AA is then metabolized via 5′-lipoxygenase to produce bioactive mediators such as leukotriene B4and 5-hydroxyeicosatetraenoate. Arachidonic acid can also be metabolized via the cyclooxygenase or prostaglandin G/H synthase (PGHS) pathway to form prostaglandins and thromboxane. We show here that human blood PMN express the PGHS 2 gene when stimulated with bacterial lipopolysaccharide (LPS). PGHS 2 mRNA increases within 30 min after LPS stimulation and PGHS 2 immunoreactive protein is detectable by 5 h. Although PGHS 1 mRNA is detectable in PMN, no immunoreactive protein is observed in either resting or LPS-stimulated cells. Following stimulation with LPS and expression of PGHS 2, PMN increase secretion of prostaglandin E2. This phenotypic change in PMN could be an important mechanism for regulating inflammation.
Details
- Title: Subtitle
- Human Neutrophils Express the Prostaglandin G/H Synthase 2 Gene When Stimulated with Bacterial Lipopolysaccharide
- Creators
- Mary B FasanoJon D WellsCharles E McCall
- Resource Type
- Journal article
- Publication Details
- Clinical immunology and immunopathology, Vol.87(3), pp.304-308
- Publisher
- Elsevier Inc
- DOI
- 10.1006/clin.1998.4545
- PMID
- 9646841
- ISSN
- 0090-1229
- eISSN
- 1090-2341
- Language
- English
- Date published
- 06/1998
- Academic Unit
- Stead Family Department of Pediatrics; Rheumatology, Allergy, and Immunology; Immunology; Internal Medicine
- Record Identifier
- 9984093331802771
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