Journal article
Human and Murine Very Small Embryonic-Like Cells Represent Multipotent Tissue Progenitors, In Vitro and In Vivo
Stem cells and development, Vol.23(7), pp.689-701
12/29/2013
DOI: 10.1089/scd.2013.0362
PMCID: PMC3967374
PMID: 24372153
Abstract
The purpose of this study was to determine the lineage progression of human and murine very small embryonic-like (HuVSEL or MuVSEL) cells in vitro and in vivo. In vitro, HuVSEL and MuVSEL cells differentiated into cells of all three embryonic germ layers. HuVSEL cells produced robust mineralized tissue of human origin compared with controls in calvarial defects. Immunohistochemistry demonstrated that the HuVSEL cells gave rise to neurons, adipocytes, chondrocytes, and osteoblasts within the calvarial defects. MuVSEL cells were also able to differentiate into similar lineages. First round serial transplants of MuVSEL cells into irradiated osseous sites demonstrated that ∼60% of the cells maintained their VSEL cell phenotype while other cells differentiated into multiple tissues at 3 months. Secondary transplants did not identify donor VSEL cells, suggesting limited self renewal but did demonstrate VSEL cell derivatives in situ for up to 1 year. At no point were teratomas identified. These studies show that VSEL cells produce multiple cellular structures in vivo and in vitro and lay the foundation for future cell-based regenerative therapies for osseous, neural, and connective tissue disorders.
Key Points
HuVSEL and MuVSEL cells are capable of differentiating into multiple germline derivatives in vitro and in vivo. MuVSEL cells have limited capacity for self-renewal and neither HuVSEL nor MuVSEL cells formed tumors in immunodeficient animals.
Details
- Title: Subtitle
- Human and Murine Very Small Embryonic-Like Cells Represent Multipotent Tissue Progenitors, In Vitro and In Vivo
- Creators
- Aaron M. Havens - University of MichiganHongli Sun - University of MichiganYusuke Shiozawa - University of MichiganYounghun Jung - Periodontics and Oral MedicineJingcheng Wang - University of MichiganAnjali Mishra - University of MichiganYajuan Jiang - NeostemDavid W. O'Neill - NeostemPaul H. Krebsbach - Biomedical Engineering - Medical SchoolDenis O. Rodgerson - NeostemRussell S. Taichman - Periodontics and Oral Medicine
- Resource Type
- Journal article
- Publication Details
- Stem cells and development, Vol.23(7), pp.689-701
- DOI
- 10.1089/scd.2013.0362
- PMID
- 24372153
- PMCID
- PMC3967374
- NLM abbreviation
- Stem Cells Dev
- ISSN
- 1547-3287
- eISSN
- 1557-8534
- Publisher
- Mary Ann Liebert, Inc
- Language
- English
- Date published
- 12/29/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
- Record Identifier
- 9984367718402771
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