Human beta-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response

Lindsey C Pingel; Karl G Kohlgraf; Christopher J Hansen; Christopher G Eastman; Deborah E Dietrich; Kindra K Burnell; Rupasree N Srikantha; Xiangjun Xiao; Myriam Bélanger; Ann Progulske-Fox; Joseph E Cavanaugh; Janet M Guthmiller; Georgia K Johnson; Sophie Joly; Zoya B Kurago; Deborah V Dawson; Kim A Brogden

doi:10.1038/icb.2008.56

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Human beta-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response

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Human beta-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response

Lindsey C Pingel, Karl G Kohlgraf, Christopher J Hansen, Christopher G Eastman, Deborah E Dietrich, Kindra K Burnell, Rupasree N Srikantha, Xiangjun Xiao, Myriam Bélanger, Ann Progulske-Fox, …

Immunology and cell biology, Vol.86(8), pp.643-649

11/2008

DOI: 10.1038/icb.2008.56

PMID: 18711400

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Abstract

Regulatory mechanisms in mucosal secretions and tissues recognize antigens and attenuate pro-inflammatory cytokine responses. Here, we asked whether human beta-defensin 3 (HBD3) serves as an upstream suppressor of cytokine signaling that binds and attenuates pro-inflammatory cytokine responses to recombinant hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis strain 381. We found that HBD3 binds to immobilized rHagB and produces a significantly higher resonance unit signal in surface plasmon resonance spectroscopic analysis, than HBD2 and HBD1 that are used as control defensins. Furthermore, we found that HBD3 significantly attenuates (P<0.05) the interleukin (IL)-6, IL-10, granulocyte macrophage colony stimulating factor (GM-CSF) and tumor-necrosis factor-alpha (TNF-alpha) responses induced by rHagB in human myeloid dendritic cell culture supernatants and the extracellular signal-regulated kinases (ERK 1/2) response in human myeloid dendritic cell lysates. Thus, HBD3 binds rHagB and this interaction may be an important initial step to attenuate a pro-inflammatory cytokine response and an ERK 1/2 response.

Recombinant Proteins - metabolism

Surface Plasmon Resonance

Cytokines - metabolism

Lectins - immunology

Dendritic Cells - immunology

Humans

Extracellular Signal-Regulated MAP Kinases

Immunity, Innate

MAP Kinase Signaling System

Adhesins, Bacterial - metabolism

Lectins - metabolism

beta-Defensins - metabolism

Adhesins, Bacterial - immunology

Porphyromonas gingivalis - immunology

Dendritic Cells - metabolism

Cytokines - immunology

Porphyromonas gingivalis - drug effects

beta-Defensins - immunology

Details

Title: Subtitle: Human beta-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response
Creators: Lindsey C Pingel - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
Karl G Kohlgraf
Christopher J Hansen
Christopher G Eastman
Deborah E Dietrich
Kindra K Burnell
Rupasree N Srikantha
Xiangjun Xiao
Myriam Bélanger
Ann Progulske-Fox
Joseph E Cavanaugh
Janet M Guthmiller
Georgia K Johnson
Sophie Joly
Zoya B Kurago
Deborah V Dawson
Kim A Brogden
Resource Type: Journal article
Publication Details: Immunology and cell biology, Vol.86(8), pp.643-649
DOI: 10.1038/icb.2008.56
PMID: 18711400
NLM abbreviation: Immunol Cell Biol
ISSN: 0818-9641
eISSN: 1440-1711
Publisher: United States
Grant note: T32 DE014678 / NIDCR NIH HHS R01 DE014390 / NIDCR NIH HHS
Language: English
Date published: 11/2008
Academic Unit: Statistics and Actuarial Science; Biostatistics; Pediatric Dentistry; Injury Prevention Research Center; Dental Research; Periodontics
Record Identifier: 9983985846702771

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