Human cDNA clones for an α subunit of Gi signal-transduction protein

P BRAY; A CARTER; V GUO; C PUCKETT; J KAMHOLZ; A SPEIGEL; M NIRENBERG

doi:10.1073/pnas.84.15.5115

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Human cDNA clones for an α subunit of Gi signal-transduction protein

Journal article

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Human cDNA clones for an α subunit of Gi signal-transduction protein

P BRAY, A CARTER, V GUO, C PUCKETT, J KAMHOLZ, A SPEIGEL and M NIRENBERG

Proceedings of the National Academy of Sciences - PNAS, Vol.84(15), pp.5115-5119

1987

DOI: 10.1073/pnas.84.15.5115

PMCID: PMC298804

PMID: 3110783

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Abstract

Two cDNA clones were obtained from a λgt11 cDNA human brain library that correspond to α(i) subunits of G signal-transduction proteins (where α(i) subunits refer to the α subunits of G proteins that inhibit adenylate cyclase). The nucleotide sequence of human brain α(i) is highly homologous to that of bovine brain α(i) [Nukada, T., Tanabe, T., Takahashi, H., Noda, M., Haga K., Haga T., Ichiyama, A., Kangawa, K., Hiranaga, M., Matsuo H. & Numa, S. (1986) FEBS Lett. 197, 305-310] and the predicted amino acid sequences are identical. However, human and bovine brain α1 cDNAs differ significantly from α(i) cDNAs from human monocytes, rat glioma, and mouse macrophages in amino acid (88% homology) and nucleotide (71-75% homology) sequences. In addition, the nucleotide sequences of the 3' untranslated regions of human and bovine brain α(i) cDNAs differ markedly from the sequences of human monocyte, rat glioma, and mouse macrophage α(i) cDNAs. These results suggest there are at least two classes of α(i) mRNA.

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Molecular Cloning

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Biological and medical sciences

Details

Title: Subtitle: Human cDNA clones for an α subunit of Gi signal-transduction protein
Creators: P BRAY - NIH, lab. molecular genetics, Bethesda MD 20892, United States
A CARTER - NIH, lab. molecular genetics, Bethesda MD 20892, United States
V GUO - NIH, lab. molecular genetics, Bethesda MD 20892, United States
C PUCKETT - NIH, lab. molecular genetics, Bethesda MD 20892, United States
J KAMHOLZ - NIH, lab. molecular genetics, Bethesda MD 20892, United States
A SPEIGEL - NIH, lab. molecular genetics, Bethesda MD 20892, United States
M NIRENBERG - NIH, lab. molecular genetics, Bethesda MD 20892, United States
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.84(15), pp.5115-5119
DOI: 10.1073/pnas.84.15.5115
PMID: 3110783
PMCID: PMC298804
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; Washington, DC
Language: English
Date published: 1987
Academic Unit: Neurology; Psychiatry
Record Identifier: 9984020731102771

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