Journal article
Human cDNA clones for four species of G alpha s signal transduction protein
Proceedings of the National Academy of Sciences - PNAS, Vol.83(23), pp.8893-8897
12/1986
DOI: 10.1073/pnas.83.23.8893
PMID: 3024154
Abstract
lambda gt11 cDNA libraries derived from human brain were screened with oligonucleotide probes for recombinants that code for alpha subunits of G signal transduction proteins. Eleven alpha s clones were detected with both probes and characterized. Four types of alpha s cDNA were cloned that differ in nucleotide sequence in the region that corresponds to amino acid residues 71-88. The clones differ in the codon for alpha s amino acid residue 71 (glutamic acid or aspartic acid), the presence or absence of codons for the next 15 amino acid residues, and the presence or absence of an adjacent serine residue. S1 nuclease protection experiments revealed at least two forms of alpha s mRNA. A mechanism for generating four species of alpha s mRNA by alternative splicing of precursor RNA is proposed.
Details
- Title: Subtitle
- Human cDNA clones for four species of G alpha s signal transduction protein
- Creators
- P BrayA CarterC SimonsV GuoC PuckettJ KamholzA SpiegelM Nirenberg
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.83(23), pp.8893-8897
- DOI
- 10.1073/pnas.83.23.8893
- PMID
- 3024154
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences; United States
- Language
- English
- Date published
- 12/1986
- Academic Unit
- Neurology; Psychiatry; Emergency Medicine
- Record Identifier
- 9984020631002771
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