Human coronavirus 229E infects polarized airway epithelia from the apical surface

Guoshun Wang; Camille Deering; Michael Macke; Jianqiang Shao; Royce Burns; Dianna M Blau; Kathryn V Holmes; Beverly L Davidson; Stanley Perlman; Paul B McCray Jr

doi:10.1128/JVI.74.19.9234-9239.2000

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Human coronavirus 229E infects polarized airway epithelia from the apical surface

Journal article

Open access

Peer reviewed

Human coronavirus 229E infects polarized airway epithelia from the apical surface

Guoshun Wang, Camille Deering, Michael Macke, Jianqiang Shao, Royce Burns, Dianna M Blau, Kathryn V Holmes, Beverly L Davidson, Stanley Perlman and Paul B McCray Jr

Journal of virology, Vol.74(19), pp.9234-9239

10/2000

DOI: 10.1128/JVI.74.19.9234-9239.2000

PMCID: PMC102122

PMID: 10982370

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Abstract

Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to the apical surface, we investigated the process of infection of airway epithelial cells by human coronavirus 229E (HCoV-229E), a common cause of respiratory tract infections. Using immunohistochemistry, we found the receptor for HCoV-229E (CD13 or aminopeptidase N) localized mainly to the apical surface of airway epithelia. When HCoV-229E was applied to the apical or basolateral surface of well-differentiated primary cultures of human airway epithelia, infection primarily occurred from the apical side. Similar results were noted when the virus was applied to cultured human tracheal explants. Newly synthesized virions were released mainly to the apical side. Thus, HCoV-229E preferentially infects human airway epithelia from the apical surface. The spike glycoprotein that mediates HCoV-229E binding and fusion to CD13 is a candidate for pseudotyping retroviral envelopes or modifying other viral vectors.

Cell Line

Cell Polarity

Coronavirus Infections - virology

Virus Replication

Coronavirus 229E, Human

Epithelial Cells - virology

Humans

Coronavirus Infections - pathology

Epithelial Cells - pathology

Coronavirus - physiology

Trachea - virology

Details

Title: Subtitle: Human coronavirus 229E infects polarized airway epithelia from the apical surface
Creators: Guoshun Wang - Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Camille Deering
Michael Macke
Jianqiang Shao
Royce Burns
Dianna M Blau
Kathryn V Holmes
Beverly L Davidson - Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania
Stanley Perlman
Paul B McCray Jr
Resource Type: Journal article
Publication Details: Journal of virology, Vol.74(19), pp.9234-9239
DOI: 10.1128/JVI.74.19.9234-9239.2000
PMID: 10982370
PMCID: PMC102122
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: HL51670-05 / NHLBI NIH HHS TPM 97-010 / HSRD VA P01 HL051670 / NHLBI NIH HHS P30 DK-97-010 / NIDDK NIH HHS R01 AI026075 / NIAID NIH HHS R01HL61460 / NHLBI NIH HHS
Language: English
Date published: 10/2000
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777345502771

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