Journal article
Human eHAND, but not dHAND, is Down-regulated in Cardiomyopathies
Journal of molecular and cellular cardiology, Vol.33(9), pp.1607-1614
09/2001
DOI: 10.1006/jmcc.2001.1434
PMID: 11549340
Abstract
The progression of cardiomyopathy to congestive heart failure is often associated with the expression of fetal cardiac-specific genes. In mice, the basic helix-loop-helix transcription factors, dHAND and eHAND, are expressed in a cardiac chamber-specific fashion and are essential for fetal cardiac development, but are down-regulated in the adult. Their expression in specific chambers of healthy and diseased human hearts has not been studied previously. HumandHAND and eHAND were mapped to human chromosomes 4q33 and 5q33, respectively, by fluorescent in situ hybridization. RNA from the four chambers of healthy human adult hearts, and from hearts of patients with several forms of cardiomyopathy, was obtained and assayed for dHAND and eHAND expression. Unlike in mice, dHAND expression was observed in all four chambers of the healthy human adult heart, but was diminished in the right atrium. In contrast,eHAND was expressed in the right and left ventricles, but was downregulated in both atrial chambers. We examined tissue from 15 human cardiomyopathic hearts obtained during cardiac transplantation or by endomyocardial biopsy for alterations inHAND gene expression. dHAND expression was unchanged in all forms of cardiomyopathy tested. However, cardiac expression of eHAND was severely down-regulated in six of six patients with ischemic cardiomyopathy and six of six patients with dilated cardiomyopathy. This study demonstrates that human dHAND and eHAND have unique spatial patterns of expression within human cardiac chambers. Downregulation of eHAND in ischemic and dilated cardiomyopathy suggests a correlation between eHAND dysregulation and the evolution of a subset of cardiomyopathies.
Details
- Title: Subtitle
- Human eHAND, but not dHAND, is Down-regulated in Cardiomyopathies
- Creators
- Aruna Natarajan - Division of Intensive Care, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, JapanHiroyuki Yamagishi - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Intensive Cardiology, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, JapanFerhaan Ahmad - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Internal Medicine, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, JapanDuanxiang Li - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Internal Medicine, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, JapanRobert Roberts - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Internal Medicine, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, JapanRumiko Matsuoka - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Department of Pediatric Cardiology, Tokyo Women's Medical University Tokyo, JapanSandra Hill - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Intensive Cardiology, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, JapanDeepak Srivastava - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Intensive Cardiology, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9148, USA, Division of Cardiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA, Tokyo Women's Medical University Tokyo, Japan
- Resource Type
- Journal article
- Publication Details
- Journal of molecular and cellular cardiology, Vol.33(9), pp.1607-1614
- Publisher
- Elsevier Ltd
- DOI
- 10.1006/jmcc.2001.1434
- PMID
- 11549340
- ISSN
- 0022-2828
- eISSN
- 1095-8584
- Language
- English
- Date published
- 09/2001
- Academic Unit
- Radiology; Molecular Physiology and Biophysics; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025434102771
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