Journal article
Human gamma enolase: isolation of a cDNA clone and expression in normal and tumor tissues of human origin
Journal of neuroscience research, Vol.19(4), pp.450-456
04/1988
DOI: 10.1002/jnr.490190409
PMID: 3385803
Abstract
We have isolated and sequenced a cDNA clone encoding the human gamma enolase. Comparison of our cDNA sequence and the rat gamma enolase sequence revealed 97% homology at the level of amino acid sequence. The two coding regions were 91% homologous on the nucleotide level, whereas the 3' noncoding regions were much less homologous (32%). Further comparison of our cDNA sequence with the human alpha enolase revealed an 82% homology at the amino acid level and a 75% homology at the nucleotide level for the two coding regions, whereas the 3' nontranslated regions were only 30% homologous. Using a portion of the 3' nontranslated region of our cDNA, shown to be specific for human gamma enolase, a single 2.5 kb mRNA was detected in human brain tissue. This same gamma enolase message was also found in a number of human normal nonneuronal tissues, and in several human tumor-derived cell lines. Expression of the mRNA for the gamma enolase subunit should thus be used with caution when identifying the cells of neuronal or neuroendocrine origin.
Details
- Title: Subtitle
- Human gamma enolase: isolation of a cDNA clone and expression in normal and tumor tissues of human origin
- Creators
- E Van Obberghen - Laboratory of Molecular Genetics, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892J KamholzJ G Bishop IIIC Zomzely-NeurathR A Lazzarini
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience research, Vol.19(4), pp.450-456
- Publisher
- United States
- DOI
- 10.1002/jnr.490190409
- PMID
- 3385803
- ISSN
- 0360-4012
- eISSN
- 1097-4547
- Language
- English
- Date published
- 04/1988
- Academic Unit
- Neurology; Psychiatry
- Record Identifier
- 9984020720202771
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