Journal article
Human glioblastoma U87MG cells transduced with a dominant negative p53 (TP53) adenovirus construct undergo radiation-induced mitotic catastrophe
Radiation research, Vol.168(2), pp.183-192
08/2007
DOI: 10.1667/0033-7587(2007)168[183:HGUCTW]2.0.CO;2
PMID: 17638400
Abstract
Human gliomas are among the most aggressive tumors, and they respond poorly to treatment. The efficacy of surgical, radiation and chemotherapy treatment of these tumors is limited by the development of resistance. Interventions aimed at altering the response of these tumors to radiation or chemotherapy treatments are needed to improve survival rate and prognosis. Glioblastomas are generally p53 (TP53) functional tumors; however, DNA repair pathways are activated in these tumors instead of the pathways to apoptosis. Thus resistance to treatment is seen in the ability of these tumors to overcome cell death. We present data that demonstrate that U87MG glioblastoma cells transduced with a dominant-negative p53 adenovirus construct become sensitized to radiation-induced mitotic catastrophe through abrogation of G(2)/M checkpoint control and overaccumulation of cyclin B1. These findings suggest that interventions abrogating the G(2)/M checkpoint sensitize these cells to radiation-induced mitotic catastrophe and may represent a novel mechanism to increase the efficacy of radiation in wild-type p53 gliomas that are resistant to apoptosis.
Details
- Title: Subtitle
- Human glioblastoma U87MG cells transduced with a dominant negative p53 (TP53) adenovirus construct undergo radiation-induced mitotic catastrophe
- Creators
- Fiorenza Ianzini - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA. fiorenza-ianzini@uiowa.eduFrederick E DomannElizabeth A KosmacekStacia L PhillipsMichael A Mackey
- Resource Type
- Journal article
- Publication Details
- Radiation research, Vol.168(2), pp.183-192
- Publisher
- United States
- DOI
- 10.1667/0033-7587(2007)168[183:HGUCTW]2.0.CO;2
- PMID
- 17638400
- ISSN
- 0033-7587
- eISSN
- 1938-5404
- Grant note
- CA58648 / NCI NIH HHS CA/GM94801 / NCI NIH HHS CA73612 / NCI NIH HHS CA66081 / NCI NIH HHS CA74899 / NCI NIH HHS
- Language
- English
- Date published
- 08/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Microbiology and Immunology; Anatomy and Cell Biology; Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984047983002771
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