Human hepatic microsomal sulfatase catalyzes the hydrolysis of polychlorinated biphenyl sulfates: A potential mechanism for retention of hydroxylated PCBs

Michael W Duffel; Kristopher Tuttle; Hans-Joachim Lehmler; Larry W Robertson

doi:10.1016/j.etap.2021.103757

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Human hepatic microsomal sulfatase catalyzes the hydrolysis of polychlorinated biphenyl sulfates: A potential mechanism for retention of hydroxylated PCBs

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Human hepatic microsomal sulfatase catalyzes the hydrolysis of polychlorinated biphenyl sulfates: A potential mechanism for retention of hydroxylated PCBs

Michael W Duffel, Kristopher Tuttle, Hans-Joachim Lehmler and Larry W Robertson

Environmental toxicology and pharmacology, Vol.88, pp.103757-103757

11/2021

DOI: 10.1016/j.etap.2021.103757

PMCID: PMC8595862

PMID: 34688910

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https://www.ncbi.nlm.nih.gov/pmc/articles/8595862View

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Abstract

Polychlorinated biphenyls (PCBs) are persistent environmental contaminants that continue to be of concern due to their varied toxicities. Upon human exposure, many PCBs with lower numbers of chlorine atoms are metabolized to hydroxylated derivatives (OH-PCBs), and cytosolic sulfotransferases can subsequently catalyze the formation of PCB sulfates. Recent studies have indicated that PCB sulfates bind reversibly with a high affinity to human serum proteins, and that they are also taken up by cells and tissues. Since PCB sulfates might be hydrolyzed to the more toxic OH-PCBs, we have investigated the ability of human hepatic microsomal sulfatase to catalyze this reaction. Twelve congeners of PCB sulfates were substrates for the microsomal sulfatase with catalytic rates exceeding that of dehydroepiandrosterone sulfate as a comparison substrate for steroid sulfatase (STS). These results are consistent with an intracellular mechanism for sulfation and de-sulfation that may contribute to retention and increased time of exposure to OH-PCBs. [Display omitted] •Human hepatic microsomes catalyze the hydrolysis of PCB sulfates.•Catalytic efficiency of the sulfatase depends upon the structure of the PCB sulfate.•No apparent gender difference was observed for pooled microsomal preparations.•A cycle of sulfation and de-sulfation may lead to the retention of toxic OH-PCBs.

Human microsomal sulfatase

Hydroxylated polychlorinated biphenyls

OH-PCB

PCB

PCB sulfate

Polychlorinated biphenyls

Steroid sulfatase

STS

Sulfotransferase

Synthesis Core

ISRP Project 1 2015-2020

Details

Title: Subtitle: Human hepatic microsomal sulfatase catalyzes the hydrolysis of polychlorinated biphenyl sulfates: A potential mechanism for retention of hydroxylated PCBs
Creators: Michael W Duffel - University of Iowa, Medicinal and Natural Products Chemistry
Kristopher Tuttle - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, IA, USA
Hans-Joachim Lehmler - University of Iowa, Occupational and Environmental Health
Larry W Robertson - University of Iowa, Occupational and Environmental Health
Resource Type: Journal article
Publication Details: Environmental toxicology and pharmacology, Vol.88, pp.103757-103757
DOI: 10.1016/j.etap.2021.103757
PMID: 34688910
PMCID: PMC8595862
NLM abbreviation: Environ Toxicol Pharmacol
ISSN: 1382-6689
eISSN: 1872-7077
Publisher: Elsevier B.V
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: P30 ES005605, P42 ES013661; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences
Language: English
Date published: 11/2021
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Iowa Superfund Research Program; Medicinal and Natural Products Chemistry
Record Identifier: 9984187039702771

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