Human leukocytes kill varicella-zoster virus-infected fibroblasts in the presence of murine monoclonal antibodies to virus-specific glycoproteins

Masahiro Ito; Toshiaki Ihara; Charles Grose; Stuart Starr

doi:10.1128/JVI.54.1.98-103.1985

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Human leukocytes kill varicella-zoster virus-infected fibroblasts in the presence of murine monoclonal antibodies to virus-specific glycoproteins

Journal article

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Human leukocytes kill varicella-zoster virus-infected fibroblasts in the presence of murine monoclonal antibodies to virus-specific glycoproteins

Masahiro Ito, Toshiaki Ihara, Charles Grose and Stuart Starr

Journal of virology, Vol.53(4), pp.98-103

1985

DOI: 10.1128/JVI.54.1.98-103.1985

PMCID: PMC254766

PMID: 2983124

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Abstract

Seven murine monoclonal antibodies reacting with major glycoproteins of varicella-zoster virus were tested for functional activity in assays for antibody-dependent cellular cytotoxicity (ADCC) and antibody-plus-complement-mediated lysis. Human peripheral blood mononuclear cells killed varicella-zoster virus-infected fibroblasts in the presence of three of four monoclonal antibodies directed against gp98/62 and a single monoclonal antibody directed against gp118. Neither of two monoclonal antibodies directed against gp66 was able to mediate ADCC. In 18-h assays, adherent effector cells were more active than nonadherent effector cells in mediating ADCC. Adherent cells treated with anti-Leu-11b and complement retained their cytotoxic activity, suggesting that monocytes are responsible for most of the adherent-cell-mediated cytotoxicity. Both immunoglobulin G1 and G2a murine monoclonal antibodies were able to participate in ADCC. Of the two immunoglobulin G2a monoclonal antibodies tested, both of which reacted with gp98/62, only one mediated lysis in the presence of complement. These results indicate that some murine monoclonal antibodies against major glycoproteins of varicella-zoster virus have functional activity in cytotoxicity assays.

Microbiology

Virology

Fundamental and applied biological sciences. Psychology

Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains

Biological and medical sciences

Details

Title: Subtitle: Human leukocytes kill varicella-zoster virus-infected fibroblasts in the presence of murine monoclonal antibodies to virus-specific glycoproteins
Creators: Masahiro Ito - Children's hosp., Philadelphia PA 19104, United States
Toshiaki Ihara - Children's hosp., Philadelphia PA 19104, United States
Charles Grose - Children's hosp., Philadelphia PA 19104, United States
Stuart Starr - Children's hosp., Philadelphia PA 19104, United States
Resource Type: Journal article
Publication Details: Journal of virology, Vol.53(4), pp.98-103
Publisher: American Society for Microbiology
DOI: 10.1128/JVI.54.1.98-103.1985
PMID: 2983124
PMCID: PMC254766
ISSN: 0022-538X
eISSN: 1098-5514
Language: English
Date published: 1985
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093480702771

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