Human ornithine-δ-aminotransferase: cDNA cloning and analysis of the structural gene

Grant A Mitchell; James E Looney; Lawrence C Brody; Gary Steel; Maureen Suchanek; John F Engelhardt; Huntington F Willard; David Valle

doi:10.1016/S0021-9258(18)68219-5

Back

Human ornithine-δ-aminotransferase: cDNA cloning and analysis of the structural gene

Journal article

Open access

Peer reviewed

Human ornithine-δ-aminotransferase: cDNA cloning and analysis of the structural gene

Grant A Mitchell, James E Looney, Lawrence C Brody, Gary Steel, Maureen Suchanek, John F Engelhardt, Huntington F Willard and David Valle

The Journal of biological chemistry, Vol.263(28), pp.14288-14295

1988

DOI: 10.1016/S0021-9258(18)68219-5

PMID: 3170546

Files and links (1)

url

https://doi.org/10.1016/S0021-9258(18)68219-5View

Published (Version of record) Open Access

Abstract

Ornithine-delta-aminotransferase (OAT) is a nuclear-encoded, mitochondrial matrix enzyme which, in rat, is expressed as basal levels in most tissues but is induced in liver by high dietary protein and in kidney by estrogen and thyroxine administration. In man, the hereditary deficiency of OAT results in ornithine accumulation and the blinding disease gyrate atrophy of the choroid and retina. We cloned near full length rat and human liver OAT cDNAs and demonstrated OAT expression in a variety of tissues from each species. We mapped the human OAT structural gene to chromosome 10, cloned 40 kilobase pairs of genomic DNA containing the complete OAT structural gene, and determined its organization. It is 21 kilobase pairs in length, and contains 11 exons. Exon 2 has been absent from all cDNAs studied and was detected by homology to X-linked processed OAT pseudogenes. The 5′-flanking region of the OAT gene has features of housekeeping genes (GC enrichment and three Sp1 binding consensus sequences) and tissue-specific, inducible genes (TATA box-like element and two CCAAT boxes). A 22-base pair region of partial dyad symmetry containing homology to estrogen responsive elements overlaps the OAT transcription site. Another 5′ sequence, GTATCCTGCCCTC, is homologous to sequences in the promoter regions of the genes of three urea cycle enzymes.

Molecular Genetics

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Genes. Genome

Molecular and cellular biology

Details

Title: Subtitle: Human ornithine-δ-aminotransferase: cDNA cloning and analysis of the structural gene
Creators: Grant A Mitchell - Johns Hopkins University
James E Looney - Johns Hopkins University
Lawrence C Brody - Johns Hopkins University
Gary Steel - Johns Hopkins University
Maureen Suchanek - Johns Hopkins University
John F Engelhardt - Johns Hopkins University
Huntington F Willard - Johns Hopkins University
David Valle - Johns Hopkins University
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.263(28), pp.14288-14295
DOI: 10.1016/S0021-9258(18)68219-5
PMID: 3170546
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology
Language: English
Date published: 1988
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984284452802771

Metrics

5 Record Views

95 Times Cited - Web of Science