Journal article
Human papillomavirus prevalence and cytopathology correlation in young Ugandan women using a low-cost liquid-based Pap preparation
Diagnostic cytopathology, Vol.38(8), pp.555-563
08/2010
DOI: 10.1002/dc.21249
PMID: 19937939
Abstract
Screening for HPV-driven cervical dysplasia and neoplasia is a significant public health concern in the developing world. The purpose of this study was to use a manual, low-cost liquid-based Pap preparation to determine HPV prevalence in HIV-positive and HIV-negative young women in Kampala, Uganda and to correlate cervical cytopathology with HPV-DNA genotype. About 196 post-partum women aged 18-30 years underwent rapid HIV testing and pelvic examination. Liquid-based cervical cytology samples were processed using a low-cost manual technique. A DNA collection device was used to collect specimens for HPV genotyping. HIV and HPV prevalence was 18 and 64%, respectively. Overall, 49% of women were infected with a high-risk HPV genotype. The most common high-risk HPV genotypes were 16 (8.2%), 33 (7.7%), 35 (6.6%), 45 (5.1%), and 58 (5.1%). The prevalence of HPV 18 was 3.6%. HIV-positive women had an HPV prevalence of 86% compared to 59% in HIV-negative women (P = 0.003). The prevalence of HPV 16/18 did not differ by HIV status. HIV-positive women were infected with a significantly greater number of HPV genotypes compared to HIV-negative women. By multivariate analysis, the main risk factor for HPV infection was coinfection with HIV. HIV-positive women were four times more likely to have abnormal cytology than HIV-negative women (43% vs. 11.6%, P < 0.001). These data highlight that HIV infection is a strong risk factor for HPV infection and resultant abnormal cervical cytology. Notably, the manual low-cost liquid-based Pap preparation is practical in this setting and offers an alternate method for local studies of HPV vaccine efficacy.
Details
- Title: Subtitle
- Human papillomavirus prevalence and cytopathology correlation in young Ugandan women using a low-cost liquid-based Pap preparation
- Creators
- Janis M Taube - Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. jtaube1@jhmi.eduBetty KamiraMahnaz MotevalliClemensia NakabiitoRobert LukandeDeidra P KellyYener S ErozanPatti E GravittMegan E BureshFrancis MmiroDanstan BagendaLaura A GuayJ Brooks Jackson
- Resource Type
- Journal article
- Publication Details
- Diagnostic cytopathology, Vol.38(8), pp.555-563
- Publisher
- United States
- DOI
- 10.1002/dc.21249
- PMID
- 19937939
- ISSN
- 8755-1039
- eISSN
- 1097-0339
- Language
- English
- Date published
- 08/2010
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047788802771
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