Human placental mesenchymal stem cells of fetal origin relieves mouse pulmonary fibrosis via downregulating MyD88 and TGF-β signaling pathway

Jin Tao; Qinglun Li; Xiaowei Ma; Fei Han; Xiaoming Liu; Jun Wei; Yongzhao Zhu

doi:10.1016/j.molimm.2017.06.032.

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Journal article

Human placental mesenchymal stem cells of fetal origin relieves mouse pulmonary fibrosis via downregulating MyD88 and TGF-β signaling pathway

Jin Tao, Qinglun Li, Xiaowei Ma, Fei Han, Xiaoming Liu, Jun Wei and Yongzhao Zhu

Xi bao yu fen zi mian yi xue za zhi, Vol.32(10), pp.1347-1351

10/2016

DOI: 10.1016/j.molimm.2017.06.032.

PMID: 27667460

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Abstract

Objective To investigate the therapeutic effect and mechanism of human placental mesenchymal stem cells of fetal origin (hfPMSCs) cultured in serum-free medium on mouse pulmonary fibrosis induced by bleomycin treatment. Methods Human hfPMSCs were cultured and identified by flow cytometry. Fifteen 6-week-old male SPF C57BL/6J mice were divided into 3 groups: bleomycin treatment group, hfPMSCs transplantation group and negative control group. Pulmonary fibrosis model was induced in the mice of bleomycin treatment group and hfPMSCs transplantation group with bleomycin (1 μg/L, 50 μL) via intratracheal instillation. The mice in negative control group were instilled with PBS (50 μL) through the same manner of the other two groups. Three days post-modelling, 200 μL containing 5×10 hfPMSCs were injected into hfPMSCs transplantation group via tail vein. All the mice were sacrificed at day 21 after modeling in batch. Lung tissues were collected for analyzing the pathological changes by HE staining and Masson staining

Pregnancy

Signal Transduction

Animals

Cell- and Tissue-Based Therapy

Female

Fetus - cytology

Humans

Lung - metabolism

Lung - pathology

Male

Mesenchymal Stem Cell Transplantation

Mesenchymal Stromal Cells - cytology

Mice

Mice, Inbred C57BL

Myeloid Differentiation Factor 88 - genetics

Myeloid Differentiation Factor 88 - metabolism

Placenta - cytology

Pulmonary Fibrosis - genetics

Pulmonary Fibrosis - metabolism

Pulmonary Fibrosis - pathology

Pulmonary Fibrosis - therapy

Transforming Growth Factor beta1 - genetics

Transforming Growth Factor beta1 - metabolism

Details

Title: Subtitle: Human placental mesenchymal stem cells of fetal origin relieves mouse pulmonary fibrosis via downregulating MyD88 and TGF-β signaling pathway
Creators: Jin Tao - Ningxia Medical University
Qinglun Li - Ningxia Medical University
Xiaowei Ma - Ningxia Medical University
Fei Han - Ningxia Medical University
Xiaoming Liu - Ningxia Medical University
Jun Wei - Ningxia Medical University
Yongzhao Zhu - Ningxia Medical University
Resource Type: Journal article
Publication Details: Xi bao yu fen zi mian yi xue za zhi, Vol.32(10), pp.1347-1351
DOI: 10.1016/j.molimm.2017.06.032.
PMID: 27667460
ISSN: 1007-8738
Language: Chinese
Date published: 10/2016
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984288812902771

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