Journal article
Human respiratory syncytial virus A2 strain replicates and induces innate immune responses by respiratory epithelia of neonatal lambs
International journal of experimental pathology, Vol.90(4), pp.431-438
Received for publication: 27 August 2008 Accepted for publication: 23 November 2008
08/2009
DOI: 10.1111/j.1365-2613.2009.00643.x
PMCID: PMC2741153
PMID: 19659901
Abstract
Human respiratory syncytial virus (hRSV) is a pneumovirus that causes significant respiratory disease in premature and full-term infants. It was our hypothesis that a common strain of RSV, strain A2, would infect, cause pulmonary pathology, and alter respiratory epithelial innate immune responses in neonatal lambs similarly to RSV infection in human neonates. Newborn lambs between 2 and 3 days of age were inoculated intrabronchially with RSV strain A2. The lambs were sacrificed at days 3, 6, and 14 days postinoculation. Pulmonary lesions in the 6-day postinoculation group were typical of RSV infection including bronchiolitis with neutrophils and mild peribronchiolar interstitial pneumonia. RSV mRNA and antigen were detected by qPCR and immunohistochemistry, respectively with peak mRNA levels and antigen at day 6. Expression of surfactant proteins A and D, sheep beta-defensin-1 and thyroid transcription factor-1 mRNA were also assessed by real-time qPCR. There was a significant increase in surfactant A and D mRNA expression in RSV-infected animals at day 6 postinoculation. There were no significant changes in sheep beta-defensin-1 and thyroid transcription factor-1 mRNA expression. This study shows that neonatal lambs can be infected with RSV strain A2 and the pulmonary pathology mimics that of RSV infection in human infants thereby making the neonatal lamb a useful animal model to study disease pathogenesis and therapeutics. RSV infection induces increased expression of surfactant proteins A and D in lambs, which may also be an important feature of infection in newborn infants.
Details
- Title: Subtitle
- Human respiratory syncytial virus A2 strain replicates and induces innate immune responses by respiratory epithelia of neonatal lambs
- Creators
- Alicia Olivier - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAJack Gallup - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAMarcia M.M.A De Macedo - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USASteven M Varga - Department of Microbiology, University of Iowa, Iowa City, IA, USAMark Ackermann - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
- Resource Type
- Journal article
- Publication Details
- International journal of experimental pathology, Vol.90(4), pp.431-438
- Edition
- Received for publication: 27 August 2008 Accepted for publication: 23 November 2008
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1111/j.1365-2613.2009.00643.x
- PMID
- 19659901
- PMCID
- PMC2741153
- ISSN
- 0959-9673
- eISSN
- 1365-2613
- Number of pages
- 8
- Language
- English
- Date published
- 08/2009
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083884902771
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