Human tau becomes phosphorylated and forms filamentous deposits when overexpressed in lamprey central neurons in situ

Garth F Hall; Jun Yao; Gloria Lee

doi:10.1073/pnas.94.9.4733

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Human tau becomes phosphorylated and forms filamentous deposits when overexpressed in lamprey central neurons in situ

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Human tau becomes phosphorylated and forms filamentous deposits when overexpressed in lamprey central neurons in situ

Garth F Hall, Jun Yao and Gloria Lee

Proceedings of the National Academy of Sciences - PNAS, Vol.94(9), pp.4733-4738

04/29/1997

DOI: 10.1073/pnas.94.9.4733

PMCID: PMC20793

PMID: 9114060

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Abstract

Microinjection of plasmids encoding human tau (htau) protein into identified lamprey reticulospinal neurons (anterior bulbar cells, or ABCs) in situ induces chronic htau expression. htau protein is transported to both the axon and dendrites of expressing ABCs by mechanisms that require the C-terminal domain of htau protein but do not require directed htau mRNA transport. htau becomes phosphorylated at the PHF-1 (Ser-396/404) and TAU-1/AT8 (Ser-199/202) epitopes throughout ABCs with heavy htau accumulations; many such cells also exhibit degenerative changes, which include the development of extracellular htau deposits. Finally, expression of htau protein fused to green fluorescent protein induced the somatodendritic accumulation of filaments containing htau when examined by immunoelectron microscopy. These results suggest that chronic expression of htau in lamprey ABCs may be useful for studying cellular mechanisms underlying tau hyperphosphorylation and filament formation in vertebrate central neurons in situ.

Biological Sciences

Details

Title: Subtitle: Human tau becomes phosphorylated and forms filamentous deposits when overexpressed in lamprey central neurons in situ
Creators: Garth F Hall - Department of Biological Sciences, University of Massachusetts, Lowell, MA 01854; and
Jun Yao - Department of Biological Sciences, University of Massachusetts, Lowell, MA 01854; and
Gloria Lee - Department of Biological Sciences, University of Massachusetts, Lowell, MA 01854; and
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.94(9), pp.4733-4738
DOI: 10.1073/pnas.94.9.4733
PMID: 9114060
PMCID: PMC20793
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 04/29/1997
Academic Unit: Iowa Neuroscience Institute; Immunology; Internal Medicine
Record Identifier: 9984065479202771

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