Journal article
Human β-defensin-3 alters, but does not inhibit, the binding of Porphyromonas gingivalis haemagglutinin B to the surface of human dendritic cells
International journal of antimicrobial agents, Vol.40(1), pp.75-79
07/2012
DOI: 10.1016/j.ijantimicag.2012.03.007
PMCID: PMC3371132
PMID: 22578747
Abstract
Human β-defensin-3 (HBD3) is a small, cationic, host defence peptide with broad antimicrobial activities and diverse innate immune functions. HBD3 binds to many microbial antigens and, in this study, we hypothesised that the known binding of HBD3 to Porphyromonas gingivalis recombinant haemagglutinin B (rHagB) alters, but does not inhibit, the binding of rHagB to human dendritic cells. To test this, human myeloid dendritic cells were incubated for 5min with rHagB, HBD3+rHagB (10:1 molar ratio), HBD3 or 0.1M phosphate-buffered saline (PBS) (pH 7.2) and were then rapidly fixed and processed for confocal microscopy and ultramicrotomy. rHagB and HBD3 could be detected with primary monoclonal mouse antibody to rHagB (MoAb 1858) or polyclonal rabbit antibody to HBD3 (P241) and secondary fluorescent-labelled anti-mouse or anti-rabbit antibodies (confocal microscopy) or protein A–colloidal gold (immunoelectron microscopy). In cells incubated with rHagB only, fluorescence and protein A–colloidal gold were seen at the cell surface and throughout the cytoplasm. In cells incubated with HBD3+rHagB, fluorescence was observed only at the cell surface in a ‘string of pearls’ configuration. Overall, these results suggest that HBD3 binding to rHagB alters, but does not inhibit, the binding of rHagB to human myeloid dendritic cells.
Details
- Title: Subtitle
- Human β-defensin-3 alters, but does not inhibit, the binding of Porphyromonas gingivalis haemagglutinin B to the surface of human dendritic cells
- Creators
- Jonathan R Van Hemert - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAErica N Recker - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USADeborah Dietrich - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAAnn Progulske-Fox - Center for Molecular Microbiology and Department of Oral Biology, University of Florida, Gainesville, FL 32610, USAZoya B Kurago - Department of Oral & Maxillofacial Pathology, Radiology and Medicine, New York University College of Dentistry, New York, NY 10010, USAKatherine S Walters - Central Microscopy Research Facility, The University of Iowa, Iowa City, IA 52242, USAJoseph E Cavanaugh - Department of Biostatistics, College of Public Health, The University of Iowa, Iowa City, IA 52242, USAKim A Brogden - Department of Periodontics and Dows Institute for Dental Research, N447 DSB, College of Dentistry, 801 Newton Road, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- International journal of antimicrobial agents, Vol.40(1), pp.75-79
- DOI
- 10.1016/j.ijantimicag.2012.03.007
- PMID
- 22578747
- PMCID
- PMC3371132
- NLM abbreviation
- Int J Antimicrob Agents
- ISSN
- 0924-8579
- eISSN
- 1872-7913
- Publisher
- Elsevier B.V
- Grant note
- name: National Institute of Dental and Craniofacial Research of the US National Institutes of Health (Bethesda, MD), award: NIH/NIDCR T32 DE014678, NIH/NIDCR R01 DE014390 and DE013545
- Language
- English
- Date published
- 07/2012
- Academic Unit
- Statistics and Actuarial Science; Preventive and Community Dentistry; Biostatistics; Injury Prevention Research Center; Dental Research; Periodontics
- Record Identifier
- 9983985715402771
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