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Hydrogen peroxide and hydroxyl radical formation by methylene blue in the presence of ascorbic acid
Journal article   Peer reviewed

Hydrogen peroxide and hydroxyl radical formation by methylene blue in the presence of ascorbic acid

G. R Buettner, Th. P Doherty and Th. D Bannister
Radiation and environmental biophysics, Vol.23(4), pp.235-243
12/1984
DOI: 10.1007/BF01407595
PMID: 6093184

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Abstract

Using ESR we have demonstrated the formation of the ascorbate free radical from sodium ascorbate, methylene blue and light. In oxygen uptake experiments we have observed the production of hydrogen peroxide while spin trapping experiments have revealed the iron catalyzed production of the hydroxyl free radical in this system. The presence of this highly reactive radical suggests that it could be the radical that initiates free radical damage in this photodynamic system.

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