Journal article
Hydroxylated and sulfated metabolites of commonly observed airborne polychlorinated biphenyls display selective uptake and toxicity in N27, SH-SY5Y, and HepG2 cells
Environmental toxicology and pharmacology, Vol.62, pp.69-78
09/2018
DOI: 10.1016/j.etap.2018.06.010
PMCID: PMC6092199
PMID: 29986280
Abstract
•Phenolic and sulfated metabolites of PCBs 3, 8, 11, and 52 were studied in vitro.•The OH-PCBs displayed toxicity to N27, SHSY-5Y, and HepG2 cells.•OH-PCBs were generally more toxic than the corresponding PCBs or PCB sulfates.•4-PCB 52 sulfate, however, showed significant toxicity to neural and hepatic cells.•Mechanisms for 4-PCB 52 sulfate toxicity may require its metabolism to 4-OH-PCB 52.
Although neurotoxicity and hepatotoxicity have long been associated with exposure to polychlorinated biphenyls (PCBs), less is known about the selective toxicity of those hydroxylated PCBs (OH-PCBs) and PCB sulfates that are metabolites derived from exposure to PCBs found in indoor air. We have examined the toxicity of OH-PCBs and PCB sulfates derived from PCBs 3, 8, 11, and 52 in two neural cell lines (N27 and SH-SY5Y) and an hepatic cell line (HepG2). With the exception of a similar toxicity seen for N27 cells exposed to either OH-PCB 52 or PCB 52 sulfate, these OH-PCBs were more toxic to all three cell-types than their corresponding PCB or PCB sulfate congeners. Differences in the distribution of individual OH-PCB and PCB sulfate congeners between the cells and media, and the ability of cells to interconvert PCB sulfates and OH-PCBs, were important components of cellular sensitivity to these toxicants.
Details
- Title: Subtitle
- Hydroxylated and sulfated metabolites of commonly observed airborne polychlorinated biphenyls display selective uptake and toxicity in N27, SH-SY5Y, and HepG2 cells
- Creators
- Eric A Rodriguez - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, United StatesBrigitte C Vanle - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, United StatesJonathan A Doorn - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, United StatesHans-Joachim Lehmler - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, IA, 52242, United StatesLarry W Robertson - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, IA, 52242, United StatesMichael W Duffel - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, United States
- Resource Type
- Journal article
- Publication Details
- Environmental toxicology and pharmacology, Vol.62, pp.69-78
- DOI
- 10.1016/j.etap.2018.06.010
- PMID
- 29986280
- PMCID
- PMC6092199
- NLM abbreviation
- Environ Toxicol Pharmacol
- ISSN
- 1382-6689
- eISSN
- 1872-7077
- Publisher
- Elsevier B.V
- Grant note
- name: NIH, award: P42 ES013661; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences; name: University of Iowa Environmental Health Sciences Research Center, award: NIEHS/NIH P30 ES05605
- Language
- English
- Date published
- 09/2018
- Academic Unit
- Occupational and Environmental Health; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Iowa Superfund Research Program; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984000925702771
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