Journal article
Hyperoxia-Triggered Aversion Behavior in Drosophila Foraging Larvae Is Mediated by Sensory Detection of Hydrogen Peroxide
Journal of neurogenetics, Vol.27(4), pp.151-162
12/01/2013
DOI: 10.3109/01677063.2013.804920
PMID: 23927496
Abstract
Reactive oxygen species (ROS) in excess have been implicated in numerous chronic illnesses, including asthma, diabetes, aging, cardiovascular disease, and neurodegenerative illness. However, at lower concentrations, ROS can also serve essential routine functions as part of cellular signal transduction pathways. As products of atmospheric oxygen, ROS-mediated signals can function to coordinate external environmental conditions with growth and development. A central challenge has been a mechanistic distinction between the toxic effects of oxidative stress and endogenous ROS functions occurring at much lower concentrations. Drosophila larval aerotactic behavioral assays revealed strong developmentally regulated aversion to mild hyperoxia mediated by H
2
O
2
-dependent activation of class IV multidendritic (mdIV) sensory neurons expressing the Degenerin/epithelial Na
+
channel subunit, Pickpocket1 (PPK1). Electrophysiological recordings in foraging-stage larvae (78-84 h after egg laying [AEL]) demonstrated PPK1-dependent activation of mdIV neurons by nanomolar levels of H
2
O
2
well below levels normally associated with oxidative stress. Acute sensitivity was reduced > 100-fold during the larval developmental transition to wandering stage (> 96 h AEL), corresponding to a loss of hyperoxia aversion behavior during the same period. Degradation of endogenous H
2
O
2
by transgenic overexpression of catalase in larval epidermis caused a suppression of hyperoxia aversion behavior. Conversely, disruption of endogenous catalase activity using a UAS-Cat
RNAi
transposon resulted in an enhanced hyperoxia-aversive response. These results demonstrate an essential role for low-level endogenous H
2
O
2
as an environment-derived signal coordinating developmental behavioral transitions.
Details
- Title: Subtitle
- Hyperoxia-Triggered Aversion Behavior in Drosophila Foraging Larvae Is Mediated by Sensory Detection of Hydrogen Peroxide
- Creators
- Myung Jun Kim - Department of Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of IowaJoshua A Ainsley - Department of Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of IowaJustin W Carder - Department of Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of IowaWayne A Johnson - Department of Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of neurogenetics, Vol.27(4), pp.151-162
- Publisher
- Taylor & Francis
- DOI
- 10.3109/01677063.2013.804920
- PMID
- 23927496
- ISSN
- 0167-7063
- eISSN
- 1563-5260
- Language
- English
- Date published
- 12/01/2013
- Academic Unit
- Neurology; Molecular Physiology and Biophysics
- Record Identifier
- 9984025346702771
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