Journal article
Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves
American journal of physiology. Heart and circulatory physiology, Vol.313(6), pp.H1143-H1154
12/01/2017
DOI: 10.1152/ajpheart.00209.2017
PMCID: PMC5814654
PMID: 28842437
Abstract
During postnatal heart valve development, glycosaminoglycan (GAG)-rich valve primordia transform into stratified valve leaflets composed of GAGs, fibrillar collagen, and elastin layers accompanied by decreased cell proliferation as well as thinning and elongation. The neonatal period is characterized by the transition from a uterine environment to atmospheric O
, but the role of changing O
levels in valve extracellular matrix (ECM) composition or morphogenesis is not well characterized. Here, we show that tissue hypoxia decreases in mouse aortic valves in the days after birth, concomitant with ECM remodeling and cell cycle arrest of valve interstitial cells. The effects of hypoxia on late embryonic valve ECM composition, Sox9 expression, and cell proliferation were examined in chicken embryo aortic valve organ cultures. Maintenance of late embryonic chicken aortic valve organ cultures in a hypoxic environment promotes GAG expression, Sox9 nuclear localization, and indicators of hyaluronan remodeling but does not affect fibrillar collagen content or cell proliferation. Chronic hypoxia also promotes GAG accumulation in murine adult heart valves in vivo. Together, these results support a role for hypoxia in maintaining a primitive GAG-rich matrix in developing heart valves before birth and also in the induction of hyaluronan remodeling in adults.
Tissue hypoxia decreases in mouse aortic valves after birth, and exposure to hypoxia promotes glycosaminoglycan accumulation in cultured chicken embryo valves and adult murine heart valves. Thus, hypoxia maintains a primitive extracellular matrix during heart valve development and promotes extracellular matrix remodeling in adult mice, as occurs in myxomatous disease.
Details
- Title: Subtitle
- Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves
- Creators
- Dorothy Amofa - Cincinnati Children's Hospital Medical CenterAlexia Hulin - Children's Medical CenterYuji Nakada - The University of Texas Southwestern Medical CenterHesham A Sadek - The University of Texas Southwestern Medical CenterKatherine E Yutzey - Cincinnati Children's Hospital Medical Center
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.313(6), pp.H1143-H1154
- DOI
- 10.1152/ajpheart.00209.2017
- PMID
- 28842437
- PMCID
- PMC5814654
- NLM abbreviation
- Am J Physiol Heart Circ Physiol
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Grant note
- R01 HL094319 / NHLBI NIH HHS R01 HL131778 / NHLBI NIH HHS
- Language
- English
- Date published
- 12/01/2017
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984843251402771
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