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Hypoxia sensing in resident cardiac macrophages regulates monocyte fate specification following ischemic heart injury
Journal article   Peer reviewed

Hypoxia sensing in resident cardiac macrophages regulates monocyte fate specification following ischemic heart injury

Farid F Kadyrov, Andrew L Koenig, Junedh M Amrute, Hao Dun, Wenjun Li, Carla J Weinheimer, Jessica M Nigro, Attila Kovacs, Andrea L Bredemeyer, Steven Yang, …
Nature cardiovascular research, Vol.3(11), pp.1337-1355
11/01/2024
DOI: 10.1038/s44161-024-00553-6
PMID: 39433910

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Abstract

Myocardial infarction initiates cardiac remodeling and is central to heart failure pathogenesis. Following myocardial ischemia-reperfusion injury, monocytes enter the heart and differentiate into diverse subpopulations of macrophages. Here we show that deletion of Hif1α, a hypoxia response transcription factor, in resident cardiac macrophages led to increased remodeling and overrepresentation of macrophages expressing arginase 1 (Arg1). Arg1 macrophages displayed an inflammatory gene signature and may represent an intermediate state of monocyte differentiation. Lineage tracing of Arg1 macrophages revealed a monocyte differentiation trajectory consisting of multiple transcriptionally distinct states. We further showed that deletion of Hif1α in resident cardiac macrophages resulted in arrested progression through this trajectory and accumulation of an inflammatory intermediate state marked by persistent Arg1 expression. Depletion of the Arg1 trajectory accelerated cardiac remodeling following ischemic injury. Our findings unveil distinct trajectories of monocyte differentiation and identify hypoxia sensing as an important determinant of monocyte differentiation following myocardial infarction.
Animals Arginase - genetics Arginase - metabolism Cell Differentiation Cell Hypoxia Cell Lineage Disease Models, Animal Hypoxia-Inducible Factor 1, alpha Subunit - genetics Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Macrophages - metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Monocytes - metabolism Myocardial Infarction - genetics Myocardial Infarction - metabolism Myocardial Infarction - pathology Myocardial Reperfusion Injury - metabolism Myocardial Reperfusion Injury - pathology Myocardium - metabolism Myocardium - pathology Phenotype Signal Transduction Ventricular Remodeling - physiology

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