I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury

Rachel G Khadaroo; Spyridon Fortis; Saad Y Salim; Catherine Streutker; Thomas A Churchill; Haibo Zhang

doi:10.1371/journal.pone.0115242

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I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury

Journal article

Open access

Peer reviewed

I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury

Rachel G Khadaroo, Spyridon Fortis, Saad Y Salim, Catherine Streutker, Thomas A Churchill and Haibo Zhang

PloS one, Vol.9(12), e115242

2014

DOI: 10.1371/journal.pone.0115242

PMCID: PMC4277349

PMID: 25541714

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Abstract

Acute mesenteric ischemia (AMI) is a life-threatening condition that can result in multiple organ injury and death. A timely diagnosis and treatment would have a significant impact on the morbidity and mortality in high-risk patient population. The purpose of this study was to investigate if intestinal fatty acid binding protein (I-FABP) and α-defensins can be used as biomarkers for early AMI and resultant lung injury. C57BL/6 mice were subjected to intestinal ischemia by occlusion of the superior mesenteric artery. A time course of intestinal ischemia from 0.5 to 3 h was performed and followed by reperfusion for 2 h. Additional mice were treated with N-acetyl-cysteine (NAC) at 300 mg/kg given intraperitoneally prior to reperfusion. AMI resulted in severe intestinal injury characterized by neutrophil infiltrate, myeloperoxidase (MPO) levels, cytokine/chemokine levels, and tissue histopathology. Pathologic signs of ischemia were evident at 1 h, and by 3 h of ischemia, the full thickness of the intestine mucosa had areas of coagulative necrosis. It was noted that the levels of α-defensins in intestinal tissue peaked at 1 h and I-FABP in plasma peaked at 3 h after AMI. Intestinal ischemia also resulted in lung injury in a time-dependent manner. Pretreatment with NAC decreased the levels of intestinal α-defensins and plasma I-FABP, as well as lung MPO and cytokines. In summary, the concentrations of intestinal α-defensins and plasma I-FABP predicted intestinal ischemia prior to pathological evidence of ischemia and I-FABP directly correlated with resultant lung injury. The antioxidant NAC reduced intestinal and lung injury induced by AMI, suggesting a role for oxidants in the mechanism for distant organ injury. I-FABP and α-defensins are promising biomarkers, and may guide the treatment with antioxidant in early intestinal and distal organ injury.

Biomarkers - metabolism

Mice, Inbred C57BL

Mesenteric Ischemia - metabolism

Male

Fatty Acid-Binding Proteins - metabolism

Mesenteric Ischemia - diagnosis

Lung Injury - diagnosis

Mesenteric Ischemia - chemically induced

Animals

Lung Injury - metabolism

Mesenteric Ischemia - complications

alpha-Defensins - metabolism

Mice

Acetylcysteine - administration & dosage

Early Diagnosis

Disease Models, Animal

Details

Title: Subtitle: I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury
Creators: Rachel G Khadaroo - Department of Surgery, University of Alberta, Edmonton, Alberta, Canada; Division of Critical Care Medicine, University of Alberta, Edmonton, Alberta, Canada
Spyridon Fortis - Departments of Anesthesia, Medicine and Physiology, Keenan Research Center for Biomedical Science of St. Michael's Hospital and University of Toronto, Toronto, Ontario, Canada
Saad Y Salim - Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
Catherine Streutker - Department of Pathology, St. Michael's Hospital, Toronto, Ontario, Canada
Thomas A Churchill - Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
Haibo Zhang - Departments of Anesthesia, Medicine and Physiology, Keenan Research Center for Biomedical Science of St. Michael's Hospital and University of Toronto, Toronto, Ontario, Canada; Department of Pathology, St. Michael's Hospital, Toronto, Ontario, Canada
Resource Type: Journal article
Publication Details: PloS one, Vol.9(12), e115242
DOI: 10.1371/journal.pone.0115242
PMID: 25541714
PMCID: PMC4277349
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Grant note: Canadian Institutes of Health Research 69042-1 / Canadian Institutes of Health Research
Language: English
Date published: 2014
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
Record Identifier: 9984094564702771

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