IBMX induces calcium release from intracellular stores in rat sensory neurones

Y Usachev; A Verkhratsky

doi:10.1016/0143-4160(95)90034-9

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IBMX induces calcium release from intracellular stores in rat sensory neurones

Journal article

Peer reviewed

IBMX induces calcium release from intracellular stores in rat sensory neurones

Y Usachev and A Verkhratsky

Cell calcium (Edinburgh), Vol.17(3), pp.197-206

03/1995

DOI: 10.1016/0143-4160(95)90034-9

PMID: 7542569

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Abstract

The intracellular free calcium concentration ([Ca2+]i) was recorded from freshly isolated rat dorsal root ganglia (DRG) neurones by means of Fura-2 or Indo-1-based microfluorimetry. Extracellular application of IBMX at millimolar concentrations evoked transient elevations in [Ca2+]i. The amplitude and rate of rise of the [Ca2+]i transient increased with increasing IBMX concentration. The effects of IBMX on [Ca2+]i were not mimicked by direct manipulation of the intracellular level of cyclic nucleotides, nor incubation with dibutyryl-cAMP, cGMP or 8-bromo-cGMP; neither did treatment with forskolin induce similar [Ca2+]i transients. The IBMX-evoked [Ca2+]i elevation persisted in Ca(2+)-free extracellular solutions, suggesting its origination was from intracellular stores. Caffeine-induced depletion of internal Ca2+ pool prevented IBMX-evoked [Ca2+]i responses, and vice versa, IBMX treatment strongly reduced the amplitude of subsequent caffeine-induced [Ca2+]i elevation. Both ryanodine and procaine, agents known to interact with Ca(2+)-gated Ca2+ release channels of the intracellular endoplasmic reticulum calcium stores, effectively inhibited IBMX-induced [Ca2+]i transients. We suggest that, in addition to its known phosphodiesterase-inhibiting activity, IBMX is an effective liberator of Ca2+ from ryanodine/caffeine-sensitive internal calcium stores in neural cells.

Animals, Newborn

Neurons, Afferent - drug effects

Cyclic GMP - pharmacology

Calcium - metabolism

Colforsin - pharmacology

Endoplasmic Reticulum - metabolism

Rats

Ganglia, Spinal - cytology

1-Methyl-3-isobutylxanthine - pharmacology

Animals

Cyclic GMP - analogs & derivatives

Dibutyryl Cyclic GMP - pharmacology

Endoplasmic Reticulum - drug effects

Caffeine - pharmacology

Cell Compartmentation - drug effects

Theophylline - pharmacology

Biological Transport - drug effects

Bucladesine - pharmacology

Details

Title: Subtitle: IBMX induces calcium release from intracellular stores in rat sensory neurones
Creators: Y Usachev - Department of General Physiology of the Nervous System, Bogomoletz Institute of Physiology, Kiev, Ukraine
A Verkhratsky
Resource Type: Journal article
Publication Details: Cell calcium (Edinburgh), Vol.17(3), pp.197-206
Publisher: Netherlands
DOI: 10.1016/0143-4160(95)90034-9
PMID: 7542569
ISSN: 0143-4160
eISSN: 1532-1991
Language: English
Date published: 03/1995
Academic Unit: Iowa Neuroscience Institute; Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
Record Identifier: 9984040585102771

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