Journal article
IFN-β production by macrophages obtained from mice undergoing graft vs host disease
The Journal of immunology (1950), Vol.141(11), pp.3823-3827
12/01/1988
DOI: 10.4049/jimmunol.141.11.3823
PMID: 2846694
Abstract
We have previously shown that when splenocytes are obtained from mice undergoing graft vs host disease (GVHD), and then placed in culture, IFN-beta is produced spontaneously in supernatants without any stimulus. Thus, when B10.D2 spleen cells are injected into sublethally irradiated BALB/c recipient mice, in vitro splenic IFN-beta production is readily apparent between days 10 to 20 post-transplantation. In the present study, experiments were carried out to characterize the cell population(s) responsible for spontaneous IFN-beta production from GVHD spleen cells. Specific antibody and C lysis of selected cell types, including T cells, B cells, and NK cells, failed to abrogate in vitro IFN production. However, IFN-producing cells from GVHD splenocytes were nylon wool adherent and could be isolated using discontinuous Percoll gradient centrifugation from the upper most large cell fractions. IFN production was also partially resistant to irradiation. Using indirect immunofluorescence and flow cytometry, IFN production was shown to be associated with MAC-1 positive cells. MAC-1 negative splenocytes demonstrated no spontaneous IFN production. Treatment of GVHD spleen cells with silica, a selective toxin for phagocytic cells, resulted in a complete inhibition of IFN production. Thus, in vitro IFN production from GVHD splenocytes appeared to come from cells having a phenotype associated with macrophages. This in vitro IFN production by macrophages represents a unique aspect of GVHD which is not typically found in tissues of normal mice.
Details
- Title: Subtitle
- IFN-β production by macrophages obtained from mice undergoing graft vs host disease
- Creators
- M. G Cleveland - The University of Texas Medical Branch at GalvestonR. B Ramirez - Dassault Systèmes (France)G. R Klimpel - The University of Texas Medical Branch at Galveston
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.141(11), pp.3823-3827
- DOI
- 10.4049/jimmunol.141.11.3823
- PMID
- 2846694
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- American Association of Immunologists
- Number of pages
- 5
- Language
- English
- Date published
- 12/01/1988
- Academic Unit
- Dermatology
- Record Identifier
- 9985164051402771
Metrics
1 Record Views