Journal article
IFN-γ-mediated suppression of coronavirus replication in glial-committed progenitor cells
Virology, Vol.384(1), pp.209-215
2009
DOI: 10.1016/j.virol.2008.10.036
PMCID: PMC2779567
PMID: 19059617
Abstract
The neurotropic JHM strain of mouse hepatitis virus (JHMV) replicates primarily within glial cells following intracranial inoculation of susceptible mice, with relative sparing of neurons. This study demonstrates that glial cells derived from neural progenitor cells are susceptible to JHMV infection and that treatment of infected cells with IFN-γ inhibits viral replication in a dose-dependent manner. Although type I IFN production is muted in JHMV-infected glial cultures, IFN-β is produced following IFN-γ-treatment of JHMV-infected cells. Also, direct treatment of infected glial cultures with recombinant mouse IFN-α or IFN-β inhibits viral replication. IFN-γ-mediated control of JHMV replication is dampened in glial cultures derived from the neural progenitor cells of type I receptor knock-out mice. These data indicate that JHMV is capable of infecting glial cells generated from neural progenitor cells and that IFN-γ-mediated control of viral replication is dependent, in part, on type I IFN secretion.
Details
- Title: Subtitle
- IFN-γ-mediated suppression of coronavirus replication in glial-committed progenitor cells
- Creators
- Lucia Whitman - Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USAHaixia Zhou - Department of Microbiology, University of Iowa, College of Medicine, Iowa City, IA 52242, USAStanley Perlman - Department of Microbiology, University of Iowa, College of Medicine, Iowa City, IA 52242, USAThomas E Lane - Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA
- Resource Type
- Journal article
- Publication Details
- Virology, Vol.384(1), pp.209-215
- DOI
- 10.1016/j.virol.2008.10.036
- PMID
- 19059617
- PMCID
- PMC2779567
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: NS41249; DOI: 10.13039/100000890, name: National Multiple Sclerosis Society, award: 2864, 3847
- Language
- English
- Date published
- 2009
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777355002771
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