Journal article
IL-15 enhances the response of human gamma delta T cells to nonpeptide [correction of nonpetide] microbial antigens
The Journal of immunology (1950), Vol.160(9), pp.4322-4329
05/01/1998
DOI: 10.4049/jimmunol.160.9.4322
PMID: 9574535
Abstract
Human gamma delta T cells have the ability to rapidly expand and produce IFN-gamma in response to nonpeptide Ags of microbial pathogens, in particular a class of compounds known as the prenyl phosphates. We investigated the ability of IL-15, a T cell growth factor, to modulate prenyl phosphate-induced gamma delta T cell proliferation and cytokine production. IL-15 significantly enhanced the expansion of gamma delta T cells in the peripheral blood after stimulation in vitro with isopentenyl pyrophosphate. Moreover, using gamma delta T cell clones, we determined that IL-15-induced T cell proliferation was dependent on the IL-2R beta chain but not the IL-2R alpha chain. We therefore studied the IL-15R alpha chain expression in human gamma delta T cells in the presence or absence of nonpeptide Ags. We found IL-15R alpha mRNA expression in IL-15-stimulated and Ag-stimulated human gamma delta T cells but not in resting gamma delta T cells. Although IL-15 itself had little effect on the production of IFN-gamma, IL-15 plus IL-12 acted synergistically to augment IFN-gamma production by gamma delta T cells. Moreover, we showed that this increase in IFN-gamma could be explained by the dual activation of STAT1 and STAT4 by IL-15 and IL-12, respectively. Taken together, these results suggest that IL-15 may contribute to activation of human gamma delta T cells in the immune response to microbial pathogens.
Details
- Title: Subtitle
- IL-15 enhances the response of human gamma delta T cells to nonpeptide [correction of nonpetide] microbial antigens
- Creators
- Veronica E García - Division of Dermatology, UCLA School of Medicine, Los Angeles, CA 90095, USADenis JullienMark SongKoichi UyemuraKe ShuaiCraig T MoritaRobert L Modlin
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.160(9), pp.4322-4329
- DOI
- 10.4049/jimmunol.160.9.4322
- PMID
- 9574535
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AR40312 / NIAMS NIH HHS AI36069 / NIAID NIH HHS AI22553 / NIAID NIH HHS
- Language
- English
- Date published
- 05/01/1998
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094667102771
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