Journal article
IL-15 trans-presentation by pulmonary dendritic cells promotes effector CD8 T cell survival during influenza virus infection
The Journal of experimental medicine, Vol.207(3), pp.521-534
03/15/2010
DOI: 10.1084/jem.20091711
PMCID: PMC2839152
PMID: 20212069
Abstract
We have recently demonstrated that peripheral CD8 T cells require two separate activation hits to accumulate to high numbers in the lungs after influenza virus infection: a primary interaction with mature, antigen-bearing dendritic cells (DCs) in the lymph node, and a second, previously unrecognized interaction with MHC I–viral antigen–bearing pulmonary DCs in the lungs. We demonstrate that in the absence of lung-resident DC subsets, virus-specific CD8 T cells undergo significantly increased levels of apoptosis in the lungs; however, reconstitution with pulmonary plasmacytoid DCs and CD8α+ DCs promotes increased T cell survival and accumulation in the lungs. Further, our results show that the absence of DCs after influenza virus infection results in significantly reduced levels of IL-15 in the lungs and that pulmonary DC–mediated rescue of virus-specific CD8 T cell responses in the lungs requires trans-presentation of IL-15 via DC-expressed IL-15Rα. This study demonstrates a key, novel requirement for DC trans-presented IL-15 in promoting effector CD8 T cell survival in the respiratory tract after virus infection, and suggests that this trans-presentation could be an important target for the development of unique antiviral therapies and more effective vaccine strategies.
Details
- Title: Subtitle
- IL-15 trans-presentation by pulmonary dendritic cells promotes effector CD8 T cell survival during influenza virus infection
- Creators
- Jodi McGill - Department of Pathology, Interdisciplinary Graduate Program in Immunology and Department of Microbiology, University of Iowa, Iowa City, IA 52241Nico Van Rooijen - Department of Molecular Cell Biology, Vrije University Medical Center, 1007 MB Amsterdam, NetherlandsKevin L Legge - Department of Pathology, Interdisciplinary Graduate Program in Immunology and Department of Microbiology, University of Iowa, Iowa City, IA 52241, Department of Pathology, Interdisciplinary Graduate Program in Immunology and Department of Microbiology, University of Iowa, Iowa City, IA 52241
- Resource Type
- Journal article
- Publication Details
- The Journal of experimental medicine, Vol.207(3), pp.521-534
- DOI
- 10.1084/jem.20091711
- PMID
- 20212069
- PMCID
- PMC2839152
- ISSN
- 0022-1007
- eISSN
- 1540-9538
- Language
- English
- Date published
- 03/15/2010
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047604002771
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