IL-17A activates ERK1/2 and enhances differentiation of oligodendrocyte progenitor cells

Jane M Rodgers; Andrew P Robinson; Elen S Rosler; Karen Lariosa-Willingham; Rachael E Persons; Jason C Dugas; Stephen D Miller

doi:10.1002/glia.22783

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IL-17A activates ERK1/2 and enhances differentiation of oligodendrocyte progenitor cells

Journal article

Peer reviewed

IL-17A activates ERK1/2 and enhances differentiation of oligodendrocyte progenitor cells

Jane M Rodgers, Andrew P Robinson, Elen S Rosler, Karen Lariosa-Willingham, Rachael E Persons, Jason C Dugas and Stephen D Miller

Glia, Vol.63(5), pp.768-779

05/2015

DOI: 10.1002/glia.22783

PMCID: PMC4400118

PMID: 25557204

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Abstract

Inflammatory signals present in demyelinated multiple sclerosis lesions affect the reparative remyelination process conducted by oligodendrocyte progenitor cells (OPCs). Interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 have differing effects on the viability and growth of OPCs, however the effects of IL-17A are largely unknown. Primary murine OPCs were stimulated with IL-17A and their viability, proliferation, and maturation were assessed in culture. IL-17A-stimulated OPCs exited the cell cycle and differentiated with no loss in viability. Expression of the myelin-specific protein, proteolipid protein, increased in a cerebellar slice culture assay in the presence of IL-17A. Downstream, IL-17A activated ERK1/2 within 15 min and induced chemokine expression in 2 days. These results demonstrate that IL-17A exposure stimulates OPCs to mature and participate in the inflammatory response.

Animals

Cell Differentiation - drug effects

Cells, Cultured

Cerebellum - cytology

Cerebellum - metabolism

Cytokines - genetics

Cytokines - metabolism

Disease Models, Animal

Encephalomyelitis, Autoimmune, Experimental - chemically induced

Encephalomyelitis, Autoimmune, Experimental - pathology

Flow Cytometry

Freund's Adjuvant - toxicity

Gene Expression Regulation - drug effects

Green Fluorescent Proteins - genetics

Green Fluorescent Proteins - metabolism

In Vitro Techniques

MAP Kinase Signaling System - drug effects

Mice

Mice, Inbred C57BL

Mice, Transgenic

Myelin Proteolipid Protein - genetics

Myelin-Oligodendrocyte Glycoprotein - toxicity

Oligodendroglia - drug effects

Oligodendroglia - enzymology

Organ Culture Techniques

Peptide Fragments - toxicity

Receptors, Interleukin-17 - deficiency

Receptors, Interleukin-17 - genetics

Stem Cells - drug effects

Stem Cells - physiology

Details

Title: Subtitle: IL-17A activates ERK1/2 and enhances differentiation of oligodendrocyte progenitor cells
Creators: Jane M Rodgers - Northwestern University
Andrew P Robinson - Northwestern University
Elen S Rosler - Free Form Fibers
Karen Lariosa-Willingham - Free Form Fibers
Rachael E Persons - Medical College of Wisconsin
Jason C Dugas - Free Form Fibers
Stephen D Miller - Northwestern University
Resource Type: Journal article
Publication Details: Glia, Vol.63(5), pp.768-779
DOI: 10.1002/glia.22783
PMID: 25557204
PMCID: PMC4400118
NLM abbreviation: Glia
ISSN: 0894-1491
eISSN: 1098-1136
Grant note: R01 NS026543 / NINDS NIH HHS
Language: English
Date published: 05/2015
Academic Unit: Obstetrics and Gynecology
Record Identifier: 9984848509102771

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