Journal article
IL-18 and IL-12 signal through the NF-kappa B pathway to induce NK-1R expression on T cells
The Journal of immunology (1950), Vol.170(10), pp.5003-5007
05/15/2003
DOI: 10.4049/jimmunol.170.10.5003
PMID: 12734344
Abstract
Substance P engages the T cell neurokinin 1 receptor (NK-1R) to enhance IFN-gamma production. NK-1R on T cells is inducible. We studied mechanisms regulating T cell NK-1R expression. Murine splenocytes were cultured for 4 h with or without rIL-12 or rIL-18. Both IL-12 and IL-18 induced splenic T cells to express NK-1R transcripts. Induction was blocked by actinomycin D, but not cycloheximide, suggesting that protein synthesis was not required for initiation of NK-1R gene transcription. Inhibition of T cell NF-kappa B activation or NF-kappa B nuclear translocation also blocked NK-1R transcription. IL-12 and IL-18 strongly induce NK-1R mRNA expression in splenocytes from Stat4(-/-) mice, suggesting that the Stat4 pathway was not required for the induction of NK-1R transcription. Splenic T cells exposed to IL-12 or IL-18 in the presence of IL-10 expressed no NK-1R mRNA. However, TGF beta did not prevent NK-1R mRNA expression. Thus, IL-12 and IL-18 induce T cells to express NK-1R through NF-kappa B activation. IL-10, a regulator of the Th1 response, blocks this activation. These data further suggest that SP and NK-1R, which promote IFN-gamma synthesis, are part of the Th1 pathway of immunity.
Details
- Title: Subtitle
- IL-18 and IL-12 signal through the NF-kappa B pathway to induce NK-1R expression on T cells
- Creators
- Joel V Weinstock - University of IowaArthur Blum - University of IowaAhmed Metwali - University of IowaDavid Elliott - University of IowaRazvan Arsenescu - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.170(10), pp.5003-5007
- DOI
- 10.4049/jimmunol.170.10.5003
- PMID
- 12734344
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/15/2003
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984359799002771
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