IL-18 promotes type 1 cytokine production from NK cells and T cells in human intracellular infection

Veronica E García; Koichi Uyemura; Peter A Sieling; Maria T Ochoa; Craig T Morita; Haruki Okamura; Masashi Kurimoto; Thomas H Rea; Robert L Modlin

doi:10.4049/jimmunol.162.10.6114

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IL-18 promotes type 1 cytokine production from NK cells and T cells in human intracellular infection

Journal article

Peer reviewed

IL-18 promotes type 1 cytokine production from NK cells and T cells in human intracellular infection

Veronica E García, Koichi Uyemura, Peter A Sieling, Maria T Ochoa, Craig T Morita, Haruki Okamura, Masashi Kurimoto, Thomas H Rea and Robert L Modlin

The Journal of immunology (1950), Vol.162(10), pp.6114-6121

05/15/1999

DOI: 10.4049/jimmunol.162.10.6114

PMID: 10229854

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Abstract

We investigated the role of IL-18 in leprosy, a disease characterized by polar cytokine responses that correlate with clinical disease. In vivo, IL-18 mRNA expression was higher in lesions from resistant tuberculoid as compared with susceptible lepromatous patients, and, in vitro, monocytes produced IL-18 in response to Mycobacterium leprae. rIL-18 augmented M. leprae-induced IFN-gamma in tuberculoid patients, but not lepromatous patients, while IL-4 production was not induced by IL-18. Anti-IL-12 partially inhibited M. leprae-induced release of IFN-gamma in the presence of IL-18, suggesting a combined effect of IL-12 and IL-18 in promoting M. leprae-specific type 1 responses. IL-18 enhanced M. leprae-induced IFN-gamma production rapidly (24 h) by NK cells and in a more sustained manner (5 days) by T cells. Finally, IL-18 directly induced IFN-gamma production from mycobacteria-reactive T cell clones. These results suggest that IL-18 induces type 1 cytokine responses in the host defense against intracellular infection.

Dose-Response Relationship, Drug

Tuberculosis, Pulmonary - immunology

T-Lymphocytes - drug effects

Humans

Interleukin-12 - immunology

Leprosy - pathology

Interleukin-18 - pharmacology

Killer Cells, Natural - drug effects

Leprosy - immunology

Monocytes - immunology

Cytokines - biosynthesis

Interferon-gamma - biosynthesis

Details

Title: Subtitle: IL-18 promotes type 1 cytokine production from NK cells and T cells in human intracellular infection
Creators: Veronica E García - Division of Dermatology and Department of Microbiology and Immunology, University of California, Los Angeles, CA 90095, USA
Koichi Uyemura
Peter A Sieling
Maria T Ochoa
Craig T Morita
Haruki Okamura
Masashi Kurimoto
Thomas H Rea
Robert L Modlin
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.162(10), pp.6114-6121
DOI: 10.4049/jimmunol.162.10.6114
PMID: 10229854
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AR40312 / NIAMS NIH HHS AI36069 / NIAID NIH HHS AI22553 / NIAID NIH HHS
Language: English
Date published: 05/15/1999
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094708102771

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