IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

Xiaoyang Hua; Kelly D. Chason; Janki Y. Patel; Warren C. Naselsky; Stephen L. Tilley

doi:10.1371/journal.pone.0024947

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IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

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IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

Xiaoyang Hua, Kelly D. Chason, Janki Y. Patel, Warren C. Naselsky and Stephen L. Tilley

PloS one, Vol.6(9), pp.e24947-e24947

09/26/2011

DOI: 10.1371/journal.pone.0024947

PMCID: PMC3180290

PMID: 21966389

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Abstract

Adenosine inhalation produces immediate bronchoconstriction in asthmatics but not in normal subjects. The bronchospastic effect of adenosine is largely mediated through adenosine-induced mast cell activation, the mechanism of which is poorly understood due to limitations in culturing human primary mast cells. Here, we show that human umbilical cord blood -derived mast cells incubated with the Th2 cytokine IL-4 develop increased sensitivity to adenosine. Potentiation of anti-IgE- induced and calcium ionophore/PMA-induced degranulation was augmented in mast cells cultured with IL-4, and this effect was reduced or abolished by pre-treatment with A 2B siRNA and selective A 2B receptor antagonists, respectively. IL-4 incubation resulted in the increased expression of A 2B and reduced expression of A 2A adenosine receptors on human mast cells. These results suggest that Th2 cytokines in the asthmatic lung may alter adenosine receptor expression on airway mast cells to promote increased responsiveness to adenosine.

Biology

Medicine

Details

Title: Subtitle: IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors
Creators: Xiaoyang Hua - University of North Carolina at Chapel Hill
Kelly D. Chason - University of North Carolina at Chapel Hill
Janki Y. Patel - University of North Carolina at Chapel Hill
Warren C. Naselsky - University of North Carolina at Chapel Hill
Stephen L. Tilley - University of North Carolina at Chapel Hill
Resource Type: Journal article
Publication Details: PloS one, Vol.6(9), pp.e24947-e24947
DOI: 10.1371/journal.pone.0024947
PMID: 21966389
PMCID: PMC3180290
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Alternative title: IL-4 Enhances Mast Cell Response to Adenosine
Language: English
Date published: 09/26/2011
Academic Unit: Otolaryngology
Record Identifier: 9984312244402771

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