Journal article
IL-6-Deficient Mice Form Granulomas in Murine Schistosomiasis That Exhibit an Altered B Cell Response
Cellular immunology, Vol.188(1), pp.64-72
08/25/1998
DOI: 10.1006/cimm.1998.1351
PMID: 9743559
Abstract
IL-6 can play an important role in various biological activities. Using IL-6-deficient, 129 × C57BL/6 mice and normal littermate controls, we studied the role of IL-6 in granulomas of mice infected with schistosomiasis mansoni. Granulomas from IL-6+/+mice produced large quantities of IL-6, derived from T, B, and myeloid cells. Yet, IL-6 mutant mice generated normal-appearing granulomas of appropriate size. Multiple-parameter flow cytometric analysis of dispersed granuloma cells revealed no substantial differences. Granuloma cells and splenocytes were culturedin vitroto measure cytokine and immunoglobulin production. Compared to control cells, IL-6−/−granuloma cells secreted more IL-4, IL-5, and IL-10. However, splenocytes secreted cytokines comparably. In the IL-6−/−state, the granuloma cells released less IgE and substantially more IgM, although IgG1, IgG2a, and IgA secretion remained normal. ELISPOT assay showed that dispersed granuloma cells from IL-6-deficient animals had substantially more IgM-secreting B cells. Thus, schistosome granulomas make IL-6 that is not essential for most aspects of granuloma development. However, IL-6 deficiency results in some disturbance of granuloma cytokine and immunoglobulin expression.
Details
- Title: Subtitle
- IL-6-Deficient Mice Form Granulomas in Murine Schistosomiasis That Exhibit an Altered B Cell Response
- Creators
- Arthur M. Blum - University of IowaAhmed Metwali - University of IowaDavid Elliott - University of IowaJie Li - University of IowaMatyas Sandor - University of Wisconsin–MadisonJoel V. Weinstock - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.188(1), pp.64-72
- Publisher
- Elsevier Inc
- DOI
- 10.1006/cimm.1998.1351
- PMID
- 9743559
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 08/25/1998
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984359918102771
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