IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle

Jeanne A Powell; Jordi Molgó; Dany S Adams; Cesare Colasante; Aislinn Williams; MacKenzie Bohlen; Enrique Jaimovich

doi:10.1523/JNEUROSCI.23-23-08185.2003

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IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle

Journal article

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IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle

Jeanne A Powell, Jordi Molgó, Dany S Adams, Cesare Colasante, Aislinn Williams, MacKenzie Bohlen and Enrique Jaimovich

The Journal of neuroscience, Vol.23(23), pp.8185-92

09/10/2003

DOI: 10.1523/JNEUROSCI.23-23-08185.2003

PMCID: PMC6740683

PMID: 12967979

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Abstract

Recently, we described an inositol 1,4,5-trisphosphate (IP3) signaling system in cultured rodent skeletal muscle, triggered by high K+ and affecting gene transcription (Powell et al., 2001). Now, in a study of adult rodent skeletal muscle, using immunocytology and confocal microscopy, we have found a high level of IP3 receptor (IP3R) staining in satellite cells, which have been shown recently to contribute to nuclei in adult fibers after muscle exercise. These IP3R staining cells are positively identified as satellite cells by their position, morphology and staining with satellite-cell-specific antibodies such as desmin and neural cell adhesion molecule. IP3Rs are also localized to postsynaptic components of the neuromuscular junction (NMJ), in areas surrounding the nuclei of the motor end plate, and in perisynaptic Schwann cells, and localized close to nicotinic acetylcholine receptors of the endplate gutters. Ca2+ imaging experiments show calcium release at the motor endplate upon K+ depolarization precisely in these IP3R-rich regions. We suggest that electrical activity stimulates IP3-associated Ca2+ signals that may be involved in gene regulation in satellite cells and in elements of the NMJ, contributing both to muscle fiber growth and stabilization of the NMJ.

Schwann Cells

Pyridinium Compounds

Receptors, Nicotinic

Calcium

Calcium Channels

Quaternary Ammonium Compounds

Aniline Compounds

Neurons and Cognition

Neural Cell Adhesion Molecules

Mice, Inbred Strains

Receptors, Cytoplasmic and Nuclear

Xanthenes

Life Sciences

Animals

Neuromuscular Junction

Inositol 1,4,5-Trisphosphate Receptors

Mice

Muscle, Skeletal

Diaphragm

Potassium

Satellite Cells, Skeletal Muscle

Calcium Signaling

Fluorescent Dyes

Details

Title: Subtitle: IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle
Creators: Jeanne A Powell
Jordi Molgó - Laboratoire de neurobiologie cellulaire et moléculaire
Dany S Adams
Cesare Colasante - Laboratorio de Fisiología de La Conducta
Aislinn Williams
MacKenzie Bohlen
Enrique Jaimovich - Centro de Estudios Moleculares de la Célula
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.23(23), pp.8185-92
Publisher: Society for Neuroscience
DOI: 10.1523/JNEUROSCI.23-23-08185.2003
PMID: 12967979
PMCID: PMC6740683
ISSN: 0270-6474
eISSN: 1529-2401
Language: English
Date published: 09/10/2003
Academic Unit: Psychiatry; Iowa Neuroscience Institute
Record Identifier: 9984066386702771

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