Journal article
ISG15 counteracts Listeria monocytogenes infection
eLife, Vol.4(2015), p.4:e06848
08/11/2015
DOI: 10.7554/eLife.06848
PMCID: PMC4530601
PMID: 26259872
Abstract
ISG15 is an interferon-stimulated, linear di-ubiquitin-like protein, with anti-viral activity. The role of ISG15 during bacterial infection remains elusive. We show that ISG15 expression in nonphagocytic cells is dramatically induced upon Listeria infection. Surprisingly this induction can be type I interferon independent and depends on the cytosolic surveillance pathway, which senses bacterial DNA and signals through STING, TBK1, IRF3 and IRF7. Most importantly, we observed that ISG15 expression restricts Listeria infection in vitro and in vivo. We made use of stable isotope labeling in tissue culture (SILAC) to identify ISGylated proteins that could be responsible for the protective effect. Strikingly, infection or overexpression of ISG15 leads to ISGylation of ER and Golgi proteins, which correlates with increased secretion of cytokines known to counteract infection. Together, our data reveal a previously uncharacterized ISG15-dependent restriction of Listeria infection, reinforcing the view that ISG15 is a key component of the innate immune response.
Details
- Title: Subtitle
- ISG15 counteracts Listeria monocytogenes infection
- Creators
- Lilliana Radoshevich - Interactions Bactéries-CellulesFrancis Impens - Interactions Bactéries-CellulesDavid Ribet - Interactions Bactéries-CellulesJuan J Quereda - Interactions Bactéries-CellulesTo Nam Tham - Interactions Bactéries-CellulesMarie-Anne Nahori - Interactions Bactéries-CellulesHélène Bierne - MICrobiologie de l'ALImentation au Service de la SantéOlivier Dussurget - Interactions Bactéries-CellulesJavier Pizarro-Cerdá - Interactions Bactéries-CellulesKlaus-Peter Knobeloch - Molecular Genetics GroupPascale Cossart - Interactions Bactéries-Cellules
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.4(2015), p.4:e06848
- DOI
- 10.7554/eLife.06848
- PMID
- 26259872
- PMCID
- PMC4530601
- NLM abbreviation
- Elife
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Publisher
- eLife Sciences Publication
- Grant note
- DOI: 10.13039/100000011, name: Howard Hughes Medical Institute (HHMI), award: Senior International Research Fellow; DOI: 10.13039/501100003762, name: Institut Pasteur, award: Bourse Roux; DOI: 10.13039/501100001677, name: Institut national de la santé et de la recherche médicale, award: U604; DOI: 10.13039/501100006488, name: Institut national de la recherche agronomique, award: USC2020; DOI: 10.13039/501100001665, name: Agence Nationale de la Recherche, award: LISTRESS; PROANTILIS; DOI: 10.13039/501100001665, name: Agence Nationale de la Recherche, award: Laboratoire d'Excellence (ANR-10-LABX-62-IBEID); DOI: 10.13039/501100000781, name: European Research Council (ERC), award: 233348 MODELIST; DOI: 10.13039/501100000854, name: Human Frontier Science Program (HFSP), award: Long Term Fellow; DOI: 10.13039/100004410, name: European Molecular Biology Organization (EMBO), award: Long Term Fellow; name: Fondation Le Roch les Mousquetaires; DOI: 10.13039/501100001706, name: Louis-Jeantet Foundation
- Language
- English
- Date published
- 08/11/2015
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984025422802771
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