Journal article
Identification, Purification, and Characterization of Staphylococcal Superantigens
Methods in molecular biology (Clifton, N.J.), Vol.1396, pp.19-33
2016
DOI: 10.1007/978-1-4939-3344-0_2
PMID: 26676034
Abstract
Purifying natively produced staphylococcal superantigens is an important process in the study of these proteins, as many common methods of protein purification are affected by staphylococcal protein A contamination. Here, we describe a proven approach for identifying superantigens in vitro as well as for purifying novel superantigens both in His-tagged and native forms using modern genetic tools coupled with thin-layer isoelectric focusing.
Details
- Title: Subtitle
- Identification, Purification, and Characterization of Staphylococcal Superantigens
- Creators
- Joseph A Merriman - Department of Microbiology, University of Iowa, 51 Newton Road, Bowen Science Building 3-403, Iowa City, IA, 52242, USAPatrick M Schlievert - Department of Microbiology, University of Iowa, 51 Newton Road, Bowen Science Building 3-403, Iowa City, IA, 52242, USA. patrick-schlievert@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1396, pp.19-33
- Publisher
- United States
- DOI
- 10.1007/978-1-4939-3344-0_2
- PMID
- 26676034
- eISBN
- 9781493933440; 1493933442
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2016
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001221502771
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