Journal article
Identification and Characterization of a Novel Gene (C4orf5) Located on Human Chromosome 4q with Specific Expression in Cardiac and Skeletal Muscle
Genomics (San Diego, Calif.), Vol.70(3), pp.347-353
12/15/2000
DOI: 10.1006/geno.2000.6399
PMID: 11161785
Abstract
The loci of several genes responsible for arrhythmogenic right ventricular dysplasia (ARVD) have been mapped. Since ARVD involves the right ventricle, we sought candidate genes preferentially expressed in the right ventricle utilizing differential display polymerase chain reaction (PCR) on mRNA from the chambers of an adult human heart. PCR products were cloned, sequenced, and used to screen an adult heart cDNA library. A novel 1.3-kb cDNA (HGMW-approved symbol C4orf5) with an open reading frame of 795 bp was identified. A probe designed from the 3′ untranslated region of the 1.3-kb cDNA was hybridized to the 1.3-kb transcript and an alternatively spliced 2.5-kb transcript in the heart and skeletal muscle RNA lanes on a multitissue Northern blot. Analysis of a 39-kb partial genomic sequence identified three intronic splice sites in the 1.3-kb transcript. The gene was mapped to human chromosome 4q26–q27. Computer-based analysis indicated that this gene is novel with no known function.
Details
- Title: Subtitle
- Identification and Characterization of a Novel Gene (C4orf5) Located on Human Chromosome 4q with Specific Expression in Cardiac and Skeletal Muscle
- Creators
- Ferhaan Ahmad - Section of Cardiology, the DeBakey Heart Center, Baylor College of Medicine, Houston, Texas, 77030Oscar Gonzalez - Section of Cardiology, the DeBakey Heart Center, Baylor College of Medicine, Houston, Texas, 77030Louis Ramagli - Department of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston, TexasJingping Xu - Department of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston, TexasMichael J Siciliano - Section of Cardiology, the DeBakey Heart Center, Baylor College of Medicine, Houston, Texas, 77030Linda L Bachinski - Section of Cardiology, the DeBakey Heart Center, Baylor College of Medicine, Houston, Texas, 77030Robert Roberts - Section of Cardiology, the DeBakey Heart Center, Baylor College of Medicine, Houston, Texas, 77030
- Resource Type
- Journal article
- Publication Details
- Genomics (San Diego, Calif.), Vol.70(3), pp.347-353
- DOI
- 10.1006/geno.2000.6399
- PMID
- 11161785
- NLM abbreviation
- Genomics
- ISSN
- 0888-7543
- eISSN
- 1089-8646
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 12/15/2000
- Academic Unit
- Radiology; Molecular Physiology and Biophysics; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025591102771
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