Journal article
Identification and Expression Patterns of Members of the Protease-Activated Receptor (PAR) Gene Family During Zebrafish Development
Developmental dynamics, Vol.240(1), pp.278-287
01/2011
DOI: 10.1002/dvdy.22517
PMCID: PMC3258453
PMID: 21181945
Abstract
Protease-activated receptors (PARs) play critical roles in hemostasis in vertebrates including zebrafish. However, the zebrafish gene classification appears to be complex and the expression patterns of
par
genes are not established. Based on analyses of genomic organization, phylogenetics, protein primary structure and protein internalization, we report the identification of four zebrafish PARs:
par1, par2a, par2b, and par3
. This classification differs from one reported previously. We also show that these genes have distinct spatiotemporal expression profiles in embryos and larvae – with
par1, par2a
and
par2b
expressed maternally and ubiquitously during gastrula stages and their expression patterns refined at later stages, and
par3
expressed only in 3-day old larvae. Notably, the expression patterns of zebrafish
par1
and
par2b
resemble those of their mammalian counterparts, suggesting that receptor function is conserved among vertebrates. This conservation is supported by our findings that Par1 and Par2b are internalized following exposure to thrombin and trypsin respectively.
Details
- Title: Subtitle
- Identification and Expression Patterns of Members of the Protease-Activated Receptor (PAR) Gene Family During Zebrafish Development
- Creators
- Hui Xu - Department of Anatomy and Cell Biology, Carver College of Medicine, the University of Iowa, 51 Newton Road, Iowa city, IA 52242-1109, USANicholas Echemendia - Department of Biological Science, Vanderbilt University, 1210 Medical Research Building III, VU Station B, Box 35-1634, Nashville, TN 37235-1634, USASonghai Chen - Department of Pharmacology, Carver College of Medicine, the University of Iowa, 51 Newton Road, Iowa city, IA 52242-1109, USAFang Lin - Department of Anatomy and Cell Biology, Carver College of Medicine, the University of Iowa, 51 Newton Road, Iowa city, IA 52242-1109, USA
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.240(1), pp.278-287
- DOI
- 10.1002/dvdy.22517
- PMID
- 21181945
- PMCID
- PMC3258453
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Grant note
- R00 RR024119-05 || RR / National Center for Research Resources : NCRR
- Language
- English
- Date published
- 01/2011
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984025683002771
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