Identification and characterization of a suite of tumor targeting peptides for non-small cell lung cancer

Michael J McGuire; Bethany Powell Gray; Shunzi Li; Dorothy Cupka; Lauren Averett Byers; Lei Wu; Shaghayegh Rezaie; Ying-Horng Liu; Naveen Pattisapu; James Issac; Tsukasa Oyama; Lixia Diao; John V Heymach; Xian-Jin Xie; John D Minna; Kathlynn C Brown

doi:10.1038/srep04480

Back

Identification and characterization of a suite of tumor targeting peptides for non-small cell lung cancer

Journal article

Open access

Peer reviewed

Identification and characterization of a suite of tumor targeting peptides for non-small cell lung cancer

Michael J McGuire, Bethany Powell Gray, Shunzi Li, Dorothy Cupka, Lauren Averett Byers, Lei Wu, Shaghayegh Rezaie, Ying-Horng Liu, Naveen Pattisapu, James Issac, …

Scientific reports, Vol.4(1), 4480

03/27/2014

DOI: 10.1038/srep04480

PMCID: PMC3967199

PMID: 24670678

Files and links (1)

url

https://doi.org/10.1038/srep04480View

Published (Version of record) Open Access

Abstract

Tumor targeting ligands are emerging components in cancer therapies. Widespread use of targeted therapies and molecular imaging is dependent on increasing the number of high affinity, tumor-specific ligands. Towards this goal, we biopanned three phage-displayed peptide libraries on a series of well-defined human non-small cell lung cancer (NSCLC) cell lines, isolating 11 novel peptides. The peptides show distinct binding profiles across 40 NSCLC cell lines and do not bind normal bronchial epithelial cell lines. Binding of specific peptides correlates with onco-genotypes and activation of particular pathways, such as EGFR signaling, suggesting the peptides may serve as surrogate markers. Multimerization of the peptides results in cell binding affinities between 0.0071-40 nM. The peptides home to tumors in vivo and bind to patient tumor samples. This is the first comprehensive biopanning for isolation of high affinity peptidic ligands for a single cancer type and expands the diversity of NSCLC targeting ligands.

Drug Delivery Systems

Ligands

Phenotype

Humans

Lung Neoplasms - metabolism

Protein Multimerization

Lung Neoplasms - pathology

Peptide Library

Heterografts

Peptides - metabolism

Molecular Structure

Disease Models, Animal

Carcinoma, Non-Small-Cell Lung - pathology

Lung Neoplasms - genetics

Amino Acid Sequence

Peptides - chemistry

Carcinoma, Non-Small-Cell Lung - genetics

Carcinoma, Non-Small-Cell Lung - metabolism

Genotype

Protein Transport

Animals

Cell Surface Display Techniques

Cell Line, Tumor

Protein Binding

Mice

Cluster Analysis

Details

Title: Subtitle: Identification and characterization of a suite of tumor targeting peptides for non-small cell lung cancer
Creators: Michael J McGuire - The University of Texas Southwestern Medical Center
Bethany Powell Gray - The University of Texas Southwestern Medical Center
Shunzi Li - The University of Texas Southwestern Medical Center
Dorothy Cupka - The University of Texas Southwestern Medical Center
Lauren Averett Byers - Department of Thoracic and Head and Neck Medical Oncology University of Texas MD Anderson Cancer Center
Lei Wu - The University of Texas Southwestern Medical Center
Shaghayegh Rezaie - The University of Texas Southwestern Medical Center
Ying-Horng Liu - The University of Texas Southwestern Medical Center
Naveen Pattisapu - The University of Texas Southwestern Medical Center
James Issac - The University of Texas Southwestern Medical Center
Tsukasa Oyama - Departments of Internal Medicine, University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Dallas, TX 75390-8807, USA
Lixia Diao - Department of Thoracic and Head and Neck Medical Oncology University of Texas MD Anderson Cancer Center
John V Heymach - Department of Thoracic and Head and Neck Medical Oncology University of Texas MD Anderson Cancer Center
Xian-Jin Xie - The University of Texas Southwestern Medical Center
John D Minna - The University of Texas Southwestern Medical Center
Kathlynn C Brown - The University of Texas Southwestern Medical Center
Resource Type: Journal article
Publication Details: Scientific reports, Vol.4(1), 4480
DOI: 10.1038/srep04480
PMID: 24670678
PMCID: PMC3967199
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Publisher: England
Grant note: 1R01CA106646 / NCI NIH HHS P30 CA016672 / NCI NIH HHS P30 CA142543 / NCI NIH HHS P50 CA070907 / NCI NIH HHS 5P30CA142543 / NCI NIH HHS P50CA70907 / NCI NIH HHS R01 CA106646 / NCI NIH HHS R01 CA164447 / NCI NIH HHS
Language: English
Date published: 03/27/2014
Academic Unit: Preventive and Community Dentistry; Biostatistics; Dental Research
Record Identifier: 9983917680702771

Metrics

18 Record Views

40 Times Cited - Web of Science